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《ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE》 1999-09
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Establishment of PCR to amplify D loop and its 5' and 3' ends of porcine mitochondrial DNA 

Liu Zhonglu, Wei Hong (Center of Laboratory Animals, Third Military Medical University,Chongging, 400038)  
Objective: To establish the PCR technique for the amplification of the D loop and its 5' and 3' ends of porcine mitochondrial DNA (mtDNA). Methods: Three pairs of primers were designed to amplify the D loop and its 5' and 3' ends of mtDNA of Xishuangbanna small ear pig of the inbred stain, Guizhou Miniature fragrant pig, Guangxi Bama miniature pig, Rongchang pig, large Yorkshire pig and Landrace pig. The amplification products were detected with electrophoresis on 1.2% agarose gel. Results: Clearly visible amplification bands were obtained and the fragment length was the same as that reported previously. Conclusion: Our findings suggest that the design of the primers is reasonable and the procedures are reliable. The PCR technique we have established can be used in PCR RFLP to study of D loop of mtDNA, in SSCP to study the 5' and 3' ends of the D loop of mtDNA, in SSCP to study the 5' and 3' ends of the D loop and in direct sequencing.
【Fund】: 国家“九五”重点科技攻关项目;国家自然科学基金
【CateGory Index】: R394.3
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