Effects of ginsenoside on immunomodulation after traumatohemorrhagic shock in rats
LIN Jing-hua, CHEN Hui-sun, HU De-yao, LIU Liang-ming (Research Institute of Field surgery, Daping Hospital, Third Military Medical University, Chongqing 400042,China)
Objective To study the modulation effects of ginsenoside (GS) on the immune function in traumatohemorrhagic shock in rats. Methods The study was divided into in vivo and in vitro part. In the in vivo sutdy, the rats were pretreated with gensinoside 2.5 mg/100 g for 3 days and inflicted with traumatohemorrhagic shock. The rats were killed on the next day and the functions of their lymphocytes and macrophages were observed. In the in vitro study, the rats were inflicted with traumatohemorrhagic shock and killed on the next day. The lymphocytes in the spleen and the macrophages in the peritoneal cavity were isolated and purified. Their immune functions after the treatment of 1 to 100 μg/ml of GS were studied. Results In the in vivo study, the pretreatment of GS resulted in enhanced proliferative capacity, elevated IL-2 releasing and increased IL-2R expression in splenocytes and enhanced antigen presentation, increased TNF releasing and phagocytosis in peritoneal macrophages. In the in vitro study, administration of GS also improved the functions of splenocytes and peritoneal macrophages. Conclusion Our findings suggest that GS administration in vivo and in vitro is able to improve the immune functions of lymphocytes and macrophages in traumatohemorrhagic shock.