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《ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE》 2000-06
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Effects of ansodamine on the activation of nuclear factor kappa B in vitro

GUO Zhen hui, HONG Xin, MAO Bao ling, QIAN Gui sheng, CHEN Zheng tang (Research Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing 400037,China)  
Objective To study the effects of ansodamine (654 2) and Lipopolysaccharide (LPS) on the level of IκB and activation of nuclear factor κB(NF κB) in monocytes. Methods Monocytes collected from volenteers were cultured and divided into 3 groups. Group 1 was the normal control, group 2 consisted of monocytes stimulated with 10 μg/ml LPS and group 3 in which the monocytes were pretreated with 10 ng/ml ansodamine and stimulated with 10 μg/ml LPS. The level of IκB and the activity of NF κB in the nuclear extract of the monocytes were examined before, 0.25, 0.5, 1 and 2 h after LPS stimulation. Results In group 2, the level of IκB began to decrease 0.25 h after LPS stimulation and reached the lowest point 0.5 h after. The peak activity of NF κB was significantly higher than the pre stimulational level and the level of the normal control ( P 0.01). In group 3 with the pretreatment of ansodamine, the lowest level of IκB remained significantly higher than that of group 2 ( P 0.01) and the activity of NF κB was higher than that of the normal control but significantly lower than the activity of group 2 ( P 0.01). Conclusion LPS can induce the degradation of IκB protein and the activation of NF κB and ansodamine reduces the degradation of IκB protein and inhibits the activation of NF κB. These facts suggest that ansodamine exerts therapeutic and protective effects on the human body against the challenge of inflammation reaction syndrome and acute lung injury.
【Fund】: 国家自然科学基金重点资助项目!(39730210)
【CateGory Index】: R392.11
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