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《ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE》 2000-06
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Effects of matrine on cell morphology and related factors of hepatocellular carcinoma cell HepG_2 in vitro

SI Wei ke, XIAO Tao yuan, KANG Ge fei (Department of Clinical Laboratory Medicine, Third Military Medical University, Chongqing 400038, China)  
Objective To study the effects of matrine on the cell morphology, cell division index and the contents of alpha fetoprotein (AFP) and proliferating cell nuclear antigen (PCNA) of human hepatocellular carcinoma cell HepG 2 in vitro . Methods: The morphology of the living cells and the cells after Wright or Sudan staining was observed with an inverted and an light microscope respectively. The surface and ultrastructures of the cells were studied with scanning and transmission electron microscope. Index of cell division was obtained with light microscope. The cellular content of AFP and PCNA were measured with immunohistochemical method and image analysis. Results HepG 2 cell without the treatment of matrine showed typical characteristics of cancer cells with an index of cell division of 53.8‰. No transparent granules or vacuoles were found in the cytoplasm. Sudan staining was negative and immunohistochemistry for AFP and PCNA was positive. HepG 2 cells treated with matrine decreased in number and growed in a scattered pattern. They were pleomorphic and their cytoplasm was filled with round and transparent granules. Wright staining revealed vacuoles existed in cytoplasm, the nucleoli were smaller in size, decreased in number and even disappeared and the index of cell division was decreased. Sudan staining showed positive particles in the cytoplasm. It was found with electron microscopy that the microvilli on cell surface became shorter and thicker and even disappeared, heterochromatin was increased in the nuclei, smooth endoplasmic reticulum was expanded and fat vacuoles and autophageosomes were formed, many rough endoplasmic reticula in lamellar arrangement were found in the cytoplasm and both nuclear and cell membranes were intact. The content of AFP and PCNA was independent on the dosage and the duration of matrine administration. Conclusion Matrine can inhibit the growth and proliferation of the HepG 2 cells in vitro and weaken, even eliminate its malignant phenotype. This finding suggests that the malignant degree decreases with the administration of matrine.Consequently, matrine may be a promising agent to treat cancers.
【Key Words】： matrine hepatocellular carcinoma cell morphology electron microscope immunohistochemitry
【CateGory Index】： R73-36
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