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Extraction and hypoglycemic action of a polysaccharide from the seeds of coix lacchryma jobi var, ma- yuen

XU Zi hui, ZHOU Shi wen, HUANG Lin qing (Base of Clinical Pharmacology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China)  
Objective To study the method of extraction of coixan, a polysaccharide from the seeds of coix lacchryma jobi var, ma yuen and its hypoglycemic action in mice. Methods ①The dried coix seeds were extracted with hot water and the extract was concentrated under lower pressure to get the crude coixan. Its protein content was removed with trichloracetic acid (TCA). Then the crude coixan was dialyzed with cellulose tubings against flowing water for 7 d and chromatographed over sephadex G 75 with a column of (0-1)mol/L NaCl to obtain purified coixan. ②The model of diabetes mellitus was established with alloxan injection through the tail vein in mice and the mode of hyperglycemia with adrenaline injection in mice too. Blood samples were collected with tail vein puncture to determine blood glucose level. Results ①Refined coixan of 0.125% yielding consisted of rhamonse, arabinose, mannose, galactose and glucose with an estimated molecular weight of 1.5×10 4. ②Oral administration of coixan to normal mice showed no obvious action to decrease blood glucose level. Peritoneal administration of coixan in the dosage from 50 to 100 mg/kg was able to decrease blood glucose level significantly in normal mice, diabetic and hyperglycemic mice ( P 0.05 to P 0.01) in dosage dependent manner. Conclusion Coixan is able to decrease the blood glucose level in normal, diabetic and hyperglycemic mice and its hypoglycemic action is determined by the route of administration.
【CateGory Index】: R285.5
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