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《Acta Academiae Medicine Militaris Tertiae》 2001-07
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Expression of lipopolysaccharide binding protein mRNA in pulmonary tissues of rat with ‘two-hit’ acute lung injury and the protective effects of anisodamine in this event

HOU Yi feng, MAO Bao ling, QIAN Gui sheng, XU Jian cheng (Institute of Respiratory Diseases of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China)  
Objective To investigate the potential role of lipopolysaccharide binding protein (LBP) in rat acute lung injury following ‘two hit' and the protective effects of anisodamine. Methods The animal model of acute lung injury was established with the oleic acid (OA, 0.2 ml/kg) and lipopolysaccharide (LPS, 2 mg/kg) in Wistar rats. LBP, TNFα and IL 10 mRNA expressions in pulmonary tissues were determined with RT PCR. The pathological changes of pulmonary tissues and arterial blood gas were observed. Results LBP mRNA expression was up regulated after injection of OA alone. The expressions of TNFα and IL 10 mRNA in rat treated with OA and LPS were higher than those in OA treated rats. Anisodamine inhibited the expressions of LBP, TNFα and IL 10 mRNA, and alleviated the pulmonary tissue damage. Conclusion The up regulation of LBP after the first hit with OA is an important mechanism of increasing sensitivity to second hit with LPS, and anisodamine has a protective effect on acute lung injury through down regulation of the LBP expression in pulmonary tissues.
【Fund】: 国家自然科学基金重点资助项目 ( 39730 2 10 )
【CateGory Index】: R563.8
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