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《Acta Academiae Medicine Militaris Tertiae》 2002-07
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Consecutive culture of human dental pulp cells in vitro

HE Fei, TAN Ying hui, YANG Zheng rong (Department of Stomatology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China)  
Objective To study if human dental pulp cells can differentiate into odontoblast like cells during long term culture. Methods The fourth to tenth passaged human dental pulp cells were cultured up to 7, 14, 21 and 30 d. The cells were observed under light microscopy (LM), and transmission electron microscopy (TEM). The expression of alkaline phosphatase and type Ⅰ collagen were assayed. Results Some of the long term cultured human dental pulp cells formed mineralized nodules. The immunohistochemical study revealed that, as odontoblasts, pulp cells contributing to the nodules formation express type Ⅰ collagen and had similar ultrastructure as odontoblasts under TEM. Conclusion The results suggest that long term cultured human dental pulp cell have the calcification ability and can differentiate into odontoblast like cells. This long term culture system represents a useful in vitro model for proliferation and differentiation of pulp cells into odontoblast like cells during pulp wound healing.
【Fund】: 全军医药卫生科研基金资助项目 ( 0 1MA165)
【CateGory Index】: R329.2
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