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《Acta Academiae Medicinae Militaris Tertiae》 2003-03
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AANAT gene-engineered embryonic stem cells decrease the amyloid β secretion of APP-overexpressed PC12 cells

WU Xuan, XU Hai wei, LI Hai di, FAN Xiao tang, CAO Juan, TANG Jun (Department of Physiology, Third Military Medical University, Chongqing 400038, China)  
Objective To construct the arylalkylamin N acetyltransferase (AANAT) gene engineered ES cells and study its possible effects on therapy of Alzheimer's disease. Methods Neomycin gene was transfected into human embryonic fibroblasts (HEF) by liposome mediated method. Using this transfected HEF as feeder layer, ES cells were transfected with AANAT gene. The conditioned medium collected from AANAT gene transfected ES cells was used to culture APP overexpressed PC12 cells. Amyloid β was detected by radioimmunoassay. Results ①HEF transfected with neomycin gene could keep the undifferentiated condition of ES cells; ②The conditioned medium of AANAT gene engineered ES cells could decrease Aβ secretion. ③Melatonin had the same effect on the decrease of Aβ secretion of APP overexpressed PC12 cells. Conclusion AANAT gene engineered ES cells have been constructed successfully. These cells can obviously decrease Aβ secretion of APP overexpressed PC12 cells and may play an important role in the therapy of AD disease.
【Key Words】： melatonin Alzheimer disease engineered cells embryonic stem cells mouse
【Fund】： 国家自然科学基金资助项目 ( 3990 0 16 9 30 10 0 0 87) ;; 全军“十五”青年基金资助项目 ( 0 1Q0 99) ;; 重庆市应用基础基金资助项目 ( 2 0 0 1)
【CateGory Index】： R749.1
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【References】
Chinese Journal Full-text Database 2 Hits
1 ;Comparison of methods for culturing mouse embryonic stem cell line ES/GFP and its effect on cell proliferation and differentiation[J];Acta Academiae Medicinae Militaris Tertiae;2004-19
2 LI Da-bing 1 , TANG Jun 1 ,FAN Xiao-tang 2 ,SONG Min 3 ,XU Hai-wei 1 ,ZHOU Guang-ji 1 ,BAI Yun 3 ( 1 Department of Physiology, 2 Department of Neurobiology, 3 Department of Medical Genetics,College of Medicine,Third Military Medical University,Chongqing 400038,China);Histopathologic comparison between PS1/APP double transgenic mouse model and Aβ_(1-40)-injected rat model of Alzheimer disease[J];Acta Academiae Medicinae Militaris Tertiae;2006-16
【Citations】
Chinese Journal Full-text Database 1 Hits
1 QIU Guan ting 1, HUANG Bing 2, TANG Shi bo 1, HU Jie 1, LIU Zhi xiong 1 (1. Vitreoretinal Laboratory, Zhongshan Ophthalmic Center, Guangzhou, 510060; 2. Experimental Animal Center, Sun Yat sen University of Medical Sciences, Guangzhou 510089,;The Investigation of Cell Culture Conditions Maintained Embryonic Stem Cells as Totipotent Cells[J];ACADEMIC JOURNAL OF SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;2000-02
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 Chun-Hua Meng,Chuan-Sheng Zhang1,Na-Na Yang2,Zi-Yu Wang3,Shao-Xian Cao,Li-Xin Du4 (Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China; 1Department of Animal Science,Hebei Normal University of Science and Technology,Changli 066600,China; 2Insititute of Life Science,Taishan Medicine University,Tai’an 271000,China; 3College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China; 4Institute of Animal,Chinese Academy of Agricultural Sciences,Beijing 100094,China);Preliminary Study on Culture of Chicken Embryonic Stem Cells by Buffalo Rat Liver Cells Conditioned Medium[J];Chinese Journal of Cell Biology;2008-04
2 Wang Ying1,2,Yue Wenbin2,Chen Lina1,Zhang Yanyan3,Guan Weijun1** (1.Institute of Animal Science,Chinese Academy of Agricultural Science,Beijiang 100094; 2.Shanxi Agricultural University,Taigu,Shanxi 030801; 3.Northwestern Sci-tech University of Agriculture and Forestry,Yangling,Shaanxi 712100);Effect Factors of Isolation & Culture and Identification of Cell Biological Characteristics of Poultry Embryonic Stem Cells[J];China Poultry;2006-20
3 Dan-yah ZHU Yi-jia LOU~2 Department of Pharmacology and Toxicology,College of Pharmaceutical Scienees,Zhejiang University,Hangzhou 310031,China;Inducible effects of icariin,icaritin,and desmethylicaritin on directional differentiation of embryonic stem cells into cardiomyocytes in vitro[J];中国药理学报(英文版);2005-04
4 TAG Jing, GE Jian, HUANG Bing, ZHUO Ye-hong, CHEN Hui-yi, GUO Yan, CHEN Xi-gu (1. Zhongshan Ophthalmic Center, 2. Experimental Animal Center, SUN Yat-sen University, Guangzhou 510060, China);Comparative Study on Culture and Differentiation of Neural Stem Cells Derived from Embryonic Stem Cells and Hippocampus[J];Journal of Sun Yat-sen University (Medical Sciences);2003-03
5 SANG Run zi,HAN Jian yong,SUN Guo jie,JIN Dong hang,TIAN Shu jun (College of Animal Science and Technonlogy,Agricultural University of Hebei,Baoding,Hebei 071001,China);Isolation and Cloning of ES-like Cells of Goat[J];Chinese Journal of Veterinary;2003-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Long-xuan, ZHAO Bin, XU Zhi-en, TANG Rong-hua. Department of Neurology, The Affiliated Hospital, Guangdong Medical College, Zhanjiang 524001, China;Prepared Radix Rehmanniae protects hippocampcal neurons of rats with Alzheimer's-like disease from amyloid-β peptide1-40 induced apoptosis[J];Chinese Journal of Neuromedicine;2006-01
2 YU Shu-guang, LUO Song, HAN Ting, TANG Yong. Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China;Effect of electroacupuncture therapy on morphological synaptic plasticity in hippocanipal neurons of rats with senile dementia[J];Chinese Journal of Neuromedicine;2006-04
3 ZHANG Xiao Dong, LI Wan Hai, WANG Qian, HUANG Mao, ZHOU Li Hua, TAN Ye Xiong (Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai 200433, China) [;Protective effects of ginsenoside Rg_1, Re on cultured hippocampal and cortex neurons injury induced by β-amyloid protein[J];Academic Journal of Second Military Medical University;2000-10
4 WU Xuan, LI Hai di, LI Shu nong, XU Hai wei, XU Lin (Department of Physiology, Third Military Medical University, Chongqing 400038, China);Differentiation of mouse embryonic stem cells to neural precursor cells in vitro[J];Acta Academiae Medicine Militaris Tertiae;2001-11
5 XU Hai wei, WU Xuan, LI Hai di, FAN Xiao tang, CAO Juan,TANG Jun(Department of Physiology, Third Military Medical University, Chongqing 400038, China);Effects of fibronectin on the induction of neural precursor cells from murine embryonic stem cells[J];Acta Academiae Medicinae Militaris Tertiae;2003-03
6 YANG Pin~(1,*),DONG Ke-li~(2),ZENG Wang-yuan~(2)(1.Department of Geriatrics,Hospital of Traditional Chinese Medicine,Hangzhou 310007;2.Department of TraditionalChinese Medicine,Third Xiangya Hospital,Central South Universty,Changsha 410003,China);Effect of yizhi jiannao granule on the behavior and neuron apoptosis in SAMP/8 mice[J];Journal of Central South University(Medical Sciences);2006-01
7 GE Jian 1* , GUO Yan\+1, WANG Zhi\|chong\+1, LIU Hai\|quan\+1, YANG Zhi\|kuan\+1, LI Yong\|ping\+1, LI Shu\|nong\+2 (1\^Zhongshan Ophthalmic Center, 2\^Department of Pathophysiology,Sun Yat\|sen University of Medical Sciences,Guangzhou\ 510060,China);PRELIMINARY STUDY ON in vitro INDUCED DIFFERENTIATION OF EMBRYONIC STEM CELLS INTO NEURONS[J];Acta Anatomica Sinica;2001-04
8 Hu Haitao, Qian Yihua, Yang Jie ,Zhang Zhangjin, Wang Weixi Fang Xiangyi(Department of Anatomy, Xi'an Medical University, Xi'an 710061);EXPRESSION OF AMYLOID PRECURSOR PROTEIN IN DORSAL HIPPOCAMPAL FORMATION OF DEMENTIA MODEL IN RAT[J];Chinese Journal of Anatomy;1997-05
9 Liu Chengzheng,et al. Department of Neurology, Tianjin First Center Hospital affiliated to the Second Magical Collage,Tianjin 300192;Clinical study of senile dementia of Alzheimer's type[J];Journal of Clinical Neurology;1994-01
10 DONG Hongtao, BAI Ying. Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China;Observation of acupuncture on cholinesterase activity of hippocampus in multifactorial model of Alzheimer's Disease[J];Shanghai Medical Journal;2002-07
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 YUAN Lu,CHEN Wei-ping(Department of Histology and Embryology,Guangxi Medical University,Nanning 530021,China);Establishment of Amplification Culture System of Pancreatic Stem Cells in vitro[J];Journal of Applied Clinical Pediatrics;2007-13
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