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《Acta Academiae Medicinae Militaris Tertiae》 2003-04
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Changes of DNA methylation during differentiation of human glioma cells induced by NDGA

LU Jia you, BIAN Xiu wu, ZHAO Wen, XU Cheng ping, JIANG Xue feng (Institute of Pathology, Southwest Hospital , Third Military Medical University, Chongqing 400038, China)  
Objective To investigate the role of NDGA in changing the activity, expression of the DNA methyltransferase (DNMT) and the patterns of genome DNA methylation in two glioma lines. Methods After two malignant glioma lines CHG 5 and SHG 44 were treated by various concentrations of NDGA, their differentiation states, the patterns of genome methylation, activity of NDMT were detected by 3H labeled tracing microassay technique and RT PCR was used to measure the expression of NDMT mRNA. Results NDGA could reduce the activity of DNMT and increase the DNA methylation degree which was dose and time dependent, but could not change the expression of DNMT mRNA. Conclusion The effect of NDGA on the inhibition of DNMT activity, increase of the DNA methylation degree may be one of the most important mechanisms of NDGA inducing differentiation effect.
【Fund】: 国家自然科学基金资助项目 ( 39970 2 6 8)
【CateGory Index】: R739.4
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