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《Acta Academiae Medicinae Militaris Tertiae》 2003-17
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Tripterygium Hypoglaucum (Levl) Hutch alkaloids induce DNA strand breaks and DNA fragmentation in Jurkat T lymphoma cells

YANG Lu jun 1, Nuesse Michael 2, AO Lin 1, CAO Jia 1 (Department of Hygiene Toxicology, Third Military Medical University, Chongqing 400038, China; 2GSF National Research Center for Environment and Health, Neuherberg, Germany,D 85764)  
Objective To investigate the effects of Tripterygium Hypoglaucum (Levl) Hutch alkaloids on the nuclear DNA strand breaks and DNA fragmantation in Jurkat T lymphoma cells to understand the mechanisms of the apoptosis. Methods After Jurkat cells were induced by the alkaloids, DNA stand breaks were labeled by TUNEL assay and DNA fragmentation was analyzed by DNA content analysis. Flow cytometry was performed to determine these phenomena. Results THH alkaloids could effectively induce DNA stand breaks and DNA fragmentation. Conclusion There are great changes in nuclear DNA in the apoptosis of Jurkat T lymphoma cells induced by THH alkaloids.
【Fund】: 德国联邦教育科学研究技术部 (BMBF)基金资助项目 ;; 国家自然科学基金资助项目 (C30 10 0 2 4 1) ;; 第三军医大学中青年科研基金资助项目 ( 2 0 0 0 2 0 0 1)
【CateGory Index】: R733.1
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