Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicinae Militaris Tertiae》 2003-19
Add to Favorite Get Latest Update

Analysis of p16 hypermethylation in sputum samples from lung cancer patients

LIU Jin yi, AN Qian, SUN Wen yue, HUANG Jin feng, PAN Qin jing, CAO Jia, CHENG Shu jun, GAO Yan ning (Department of Hygiene Toxicology, College of Military Preventive Medicine, Third Military Medical University, Chongqing 400038, China)  
Objective To analyze the aberrant methylation of p16 promoter in sputum samples from lung cancer patients and to evaluate p16 hypermethylation as an effective biomarker for the early auxilliary diagnosis of lung cancer. Methods A modified semi nested methylation specific PCR (MSP) was performed for the detection of p16 hypermethylation in sputum and corresponding partial tumor tissue samples from 94 cases of lung cancer patients and 10 cases of chronic inflammation. Results The hypermethylation of p16 promoter was detected in 74% of the sputum specimens. Compared with conventional cytological analysis, detection of p16 hypermethylation in sputum samples could be a more sensitive and specific approach for the diagnosis of lung cancer. Combination of cytological analysis and detection of p16 hypermethylation in sputum could identify 86.17% (81/94) lung cancer. The patients whose sputum cells had hypermethylated p16 gene also exhibited aberrant methylation in their tumor cells. In 10 cases of chronic inflammation, p16 hypermethylation was only detected in 3 cases. Conclusion Detection of p16 hypermethylation in sputum of the lung cancer patients might offer an effective means for the earlier auxillary diagnosis of this malignancy.
【Fund】: 国家“九五”攻关项目 ( 96 90 6 0 1 14 ) ;; 国家重点基础研究发展规划资助项目 (“973”项目 ) (G19980 512 0 7)
【CateGory Index】: R734.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved