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《Acta Academiae Medicinae Militaris Tertiae》 2003-22
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Effects of TSPG combined with EPO or GM-CSF on differentiation of K562 cells

CHEN Di long, WANG Ya ping, CHEN Ting mei (Department of Histology and Embryology, Chongqing University of Medical Sciences, Chongqing 400016, China)  
Objective To investigate the effects of total saponins of Panax ginseng(TSPG) combined with erythropoietin(EPO) or granulocyte macrophage colony stimulating factor(GM CSF) on the differentiation of K562 cells. Methods Cells were cultured in vitro . Morphologic changes of cells were observed with light microscope and electron microscope. The levels of hemoglobin, peroxidase, non specific lipase α NAE in cells were detected respectively by benzidine staining, peroxidase(POX) staining and α NAE cytochemical staining. Levels of cell surface differentiation antigens CD15, HIR2, EPO R and GM CSF R were determined by immunocytochemistry. Results TSPG combined with EPO or GM CSF resulted in more mature morphological features of K562, increased expressions of hemoglobin and myeolperoxidase in K562 cells, and enhanced expressions of cell surface differentiation antigens CD15, HIR2, EPO R and GM CSF R. Conclusion TSPG combined with cytokine EPO or GM CSF can induce more mature differentiation of K562 cells.
【Fund】: 国家自然科学基金资助项目 ( 396 70 880 )~~
【CateGory Index】: R733.7
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