Functional heterogeneity of monocytes/macrophages and its significance in immune dys function after trauma-hemorrhage in rats
YIN Hua hua 1, ZHU Jing ci 1, GU Chang guo 2, MA Zheng 3, HU Cheng xiang 2, LI Lei 2 ( 1Department of Nursing, Third Military Medical University, Chongqing 400038; 2State Key Laboratory of Trauma, Burns and Combined Injury, Department 1, Institute of Surgery Research; 3Department of Thoracic Surgery, Daping Hospital, 400042, China)
Objective To investigate the relationship between the functional heterogeneity of monocytes/macrophages and the immune dysfunction after severe trauma. Methods A total of 48 healthy Wistar rats were divided into control group and post trauma hemorrhage group. The non specific phagocytotic abilities and the expression of I A of differently distributed monocytes/macrophages were evaluated by benzaldehyde green assay and flow cytometry, respectively. Results (1) In the control group, alveolar macrophages (AM), peritoneal macrophages (PM), monocytes (Mo) showed a great functional difference in phagocytosis and expression of I A; (2) After trauma hemorrhage, the nonspecific phagocytotic ability of AM, PM, and Mo isolated from the injured rats declined significantly, but that of AM decreased most significantly and lasted longer. The changes of I A antigen expression of monocytes/macrophages after trauma hemorrhage varied. The nonspecific phagocytotic ability of PM remained at a very low level after trauma, but that of AM began to increase at 4 and 7 d after trauma. Conclusion There is phenotypic and functional heterogeneity of monocytes/macrophages under normal condition. The heterogeneity is more obvious after trauma hemorrhage, which may play an important role in the immune dysfunction and the development of complications after trauma.
【CateGory Index】： R641