Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicinae Militaris Tertiae》 2004-05
Add to Favorite Get Latest Update

Effects of LOV on p21 in BRCA1-overexpressing MCF-7 cells

LUO Yuan qiong, MI Man tian, XU Hong xia, WEI Na, ZHANG Qian yong (Department of Nutrition Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China)  
Objective To investigate the effects of synthetic inhibitor of cholesterol lovastatin (LOV) on p21 in BRCA1 overexpressing MCF 7 cells. Methods MCF 7 cells were transfected with BRCA1 gene to establish BRCA1 overexpressing MCF 7 cells (MCF 7BRCA1) by gene transfection technigue. After treatment of MCF 7 cells with 8 μmol/L LOV for 1, 2, and 3 d, the cell proliferation capacity of MCR 7 and MCR 7BRCA1 was observed by MTT, and the protein expression of p21 was determined by Western blotting. Results After treatment of MCF 7 cells with LOV, the proliferation capacity decreased, but protein expression of p21 was up regulated. These changes were more significant in BRCA1 transfected cells than the nontransfected cells. Conclusion BRCA1 gene can enhance the inhibitory effect of LOV on the cell proliferation of MCF 7 cells and can induce the up regulated expression of p21, which might be one of the important molecular mechanisms of the inhibitory effect of LOV on the cell proliferation of BRCA1 overexpressing MCF 7 cells.
【Fund】: 国家自然科学基金资助项目 ( 30 2 7112 8)~~
【CateGory Index】: R737.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved