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《Acta Academiae Medicinae Militaris Tertiae》 2004-08
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Typing of Acinetobacter baumannii isolated from patients in intensive care unit with IRS-PCR

ZHANG Qing ling 1, LIU Yu fu 2, LIU Ming hua 3, WANG Xian yuan 4, FU Wei ling 3 ( 1Department of Neurology; 2Department of Nursing, Xinqiao Hospital, Third Military Medical University, Chongqing 400037; 3Department of Medical Laboratory, Southwest Hospital, Third Military Medical University, Chongqing 400038; 4Health Bureau, Health Department, General Logistics Department of PLA, Beijing 100842, China)  
Objective To type Acinetobacter baumannii using infrequent restriction site PCR (IRS PCR). Methods Strain specific electrophoretic patterns from PCR products by amplifying DNA sequences flanking infrequent restriction sites of 15 strains of Acinetobacter baumannii were compared with the results of biotyping and antimicrobial susceptibility. Results The 15 bacteria were divided into 5 gene types with IRS PCR, but 3 with biotyping, and 4 with antimicrobial susceptibility. Conclusion IRS PCR method for typing Acinetobacter baumannii is of strong sensitivity, high recognition, good repeatability, convenient operation, and wide range of application.
【CateGory Index】: R446.5
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【Co-citations】
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1 ZHANG Qing ling, LIU Ming hua, WANG Xian yuan, FU Wei ling (Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China);A clinical study of A. baumannii infection patients treated with imipenem-cilastatin and ampicillin-sulbactam[J];Acta Academiae Medicinae Militaris Tertiae;2003-07
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1 LIAN Xiang qun, LIN Ya lei, LIU Ding, YU Zhi hai, CHEN Wei (Department of Anesthesia, The 202nd Military Hospital, Shenyang 110003, China);Typing of Acinetobacter baumannii with enterbacterial repetitive intergenic consensus PCR[J];Acta Academiae Medicine Militaris Tertiae;2002-04
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