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《Acta Academiae Medicinae Militaris Tertiae》 2004-09
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Immune response of Helicobacter pylori in BALB/c mice by oral immunization with recombinant HspA

GUO Ying, ZOU Quan ming, ZU Yong hong, WU Chao, GUO Gang, MAO Xu hu, YUAN Xiao peng (Department of Clinical Microbiology and Immunology, College of Medical Laboratory, Third Military Medical University, Chongqing 400038, China)  
Objective To evaluate the immune response efficiency of recombinant HspA (rHspA) as an oral vaccine in BALB/c mice. Methods BALB/c mice were vaccinated with rHspA combined with choleratoxin (CT) or heat labile enterotoxin subunit B (LTB). The levels of specific IgG and IgA antibodies to Helicobacter pylori were determined in sera, fluids of stomach and intestine by ELISA. Proliferation of spleen lymphocytes cultured in vitro and the changes of IL 4, IFN γ, stimulated by Helicobacter pylori , were investigated by 3H TdR incorporation and ELISA. Results No significant difference in level of anti HspA antibodies between rHspA and control groups was found, but the level of antibodies in rHspA plus adjuvant group was significantly higher than that in the control group. Levels of IL 4 and IFN γ in rHspA plus adjuvant group were higher than those in the control group. In rHspA group, however, only IFN γ increased. Conclusion Oral administration of rHspA plus CT (or LTB) in BALB/c mice can stimulate local and systematic immune response, humoral immune response, and cellular immune response of the mice.
【Fund】: 国家“九五”重点科技攻关计划项目 ( 96 90 1 0 1 54 ) ;; “86 3”生物与现代农业技术领域生物工程技术主题课题 ( 2 0 0 1AA2 1516 1)~~
【CateGory Index】: R392
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1 MAO Xu hu, ZOU Quan ming, LU Dong shui, ZENG Wei kun, Guo Gang, WANG Yi Chao, XIE Qing hua (Department of Clinical Microbiology, Third Military Medical University, Chongqing 400038, China);Immune responses of Helicobacter pylori in BalB/c mice by oral immunization with subunit B of recombinant urease[J];Acta Academiae Medicine Militaris Tertiae;2002-03
2 LU Dong shui, YU Chang qing, ZOU Quang ming (Department of Clinical Microbiology,Third Military Medical University, Chongqing 400038,China);Study of gastroenteric mucosa immune responses in Balb/c mice by oral immunization with helicobacter pylori[J];Immunological Journal;2001-02
【Co-citations】
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1 MAO Xu hu, ZOU Quan ming, LU Dong shui, ZENG Wei kun, Guo Gang, WANG Yi Chao, XIE Qing hua (Department of Clinical Microbiology, Third Military Medical University, Chongqing 400038, China);Immune responses of Helicobacter pylori in BalB/c mice by oral immunization with subunit B of recombinant urease[J];Acta Academiae Medicine Militaris Tertiae;2002-03
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