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《Acta Academiae Medicinae Militaris Tertiae》 2004-09
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Effects of PI3K pathway on multicellular resistance of lung adenocarcinoma A549 cells via affecting cell apoptosis

HUANG Hai hui, LIANG Hou jie, HU Shao yi, BIAN Zhi heng, HE Jian ming (Department of Oncology, Southwest Hospital, Third Medical Military University, Chongqing 400038, China)  
Objective To investigate the roles of PI3K pathway in multicellular resistance of lung adenocarcinoma A549 cells. Methods Three dimensional culture of A549 cells was conducted using rotatable culture bottle and desk top water bath shaker. Western blot, in situ cell apoptosis detection, MTT, and blood cell counter were employed to detect the expressions of Akt, Bcl 2, and Bad, the apoptotic rate, and the susceptibility of apoptosis to adriamycin (ADM) of A549 cells under different culture conditions. Results Compared with cells cultured in two dimensional culture system (2 D system), higher Akt and Bcl 2 expressions, lower Bad expression and apoptotic rate, and decreased susceptibility to ADM were found in cells cultured in three dimensional culture system (3 D system). The differences diminished significantly after blocking the PI3K pathway. Conclusion PI3K pathway has effect on the multicellular resistance of lung adenocarcinoma A549 cells via inhibiting cell apoptosis. Blocking this pathway may revert the resistance.
【Fund】: 国家自然科学基金资助项目 ( 30 1710 6 7)~~
【CateGory Index】: R734.2
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