Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicinae Militaris Tertiae》 2004-17
Add to Favorite Get Latest Update

Effects of recombinant antisense c-myc adenovirus in induction of differentiation of HL-60 cells

CHEN Long 1, CHEN Jie ping 1, LIN Chen 2 ( 1Department of Hematology, Southwest Hospital, Third Military Medical University, Chongqing, 400038; 2Department of Cell Biology, Institute of Oncology, Chinese Academy of Medical Sciences, Beijing 100021, China)  
Objectives To evaluate the potential of antisense c myc gene therapy for acute promyelocytic leukemia by investigating the biological effects and molecular mechanisms in induction of differentiation of HL 60 cell line using recombinant antisense c myc adenovirus (Ad AS c myc). Methods Cultured HL 60 cells treated with Ad AS c myc or Ad LacZ with polybrene and protamine sulfate were analyzed by X gal staining, morphology, MTT, flow cytometric analysis, RT PCR, and immunocytochemical techniques in vitro . Results HL 60 cells could be transfected effectively by Ad LacZ+protamine sulfate (79.8%). The level of c myc transcription and the version could be strongly inhibited by Ad AS c myc in the transfected HL 60 cells. Ad AS c myc could strongly inhibit the cell growth in HL 60 cells (51%). Ad AS c myc could reduce the ratio of nuclear/cytoplasm, and increase the activity of peroxidase in HL 60 cells. Ad AS c myc could lead to the blocking of G 0/G 1 phase in HL 60 cells. Ad AS c myc could also increase the expression of c fos in HL 60 cells. Conclusion The expression of Ad AS c myc can inhibit the growth and induce differentiation of HL 60 cells in vitro . The biological effects of Ad AS c myc may be closely associated with the activity of peroxidase and c fos gene in HL 60 cells. Ad AS c myc is of clinical potential in gene thera py for acute promyelocytic leukemia.
【CateGory Index】: R733.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 YUE Bao-hong~(1,2),ZHU Xiao-yan~(2),ZHANG Gong-yuan~(2),SUN Ling~(3),ZHAO Xiao-qiang~(3),WANG Qing-xia~(1),LIU Shuai~(1),ZHANG Qiu-tang~(3),ZHANG Qin-xian~(2)(~(1)Clinical Laboratory,~(3)Department of Hematology,The First Affiliated Hospital,~(2)Department of Histology and Embryology,School of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052,China);Effect of specific short hairpin RNA of nucleostemin on differentiation antigens and biological properties of HL-60 cells[J];Acta Academiae Medicinae Militaris Tertiae;2006-11
2 ZHANG Hui1,2),YUE Baohong1,2),ZHANG Gongyuan2),YIN Lei2),LE Xiaoping2),LIU Guohong2),FENG Ruo2),DING Yi2),ZHANG Qinxian2)1)Department of Clinical Laboratory, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001;Effects of ATRA on mRNA expression of nucleostemin in HL-60 cells[J];Journal of Zhengzhou University(Medical Sciences);2008-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YANG Feng guang 1, ZHANG Zhi wen 2△ , XIN Dian qi 1, SHI Chang jin 1, WU Xue qing 1, LIU Wu jiang 1,GUO Ying lu 1, WU Jie ping 1 (1.Department of Urology,Peking University First Hospital; Peking University Institute of Urology; 2. Department of Physiology,Peking University School of Basic Medical Sciences,Beijing 100083,China);Peroxisome proliferator-actived receptor-γ ligand troglitazone induces apoptosis in renal cell carcinoma[J];Journal of Peking University(Health Sciences);2004-02
2 PEI Li 1, CHEN Jie ping 1, LIANG Hou jie 2 ( 1Department of Hematology; 2Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Expressions and activation of JAK2 and STAT5 during the process of differentiation of human cord blood CD34~+ cells into dendritic cells[J];Acta Academiae Medicinae Militaris Tertiae;2004-11
3 LI Yan 1,HUANG Jian 2,CHEN Jie ping1,et al. (1 Department of Tumor & Hematology,Southwest Hospital,the Third Military Medical University, Chongqing 400038,China;2.Yishengtang Biology Co.,Ltd.,Shenzhen,Guangdong 518026,China);Dectection of P53 and K-ras gene mutations in leukemia by oligonucleotide microarry[J];Chongqing Medical Journal;2003-12
4 WEI Li, KONG Pei-yan, CHEN Xing-hua, PENG Xian-gui, CHANG Cheng, ZENG Dong-feng, LIU Hong, LIU Lin, WANG Qing-yu, ZHANG Yi (Department of Hematology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China);Effects of inhibition of SDF-1 activity in normal stromal cells on biological properties of human acute myelocytic leukemia cell line HL-60[J];Acta Academiae Medicinae Militaris Tertiae;2004-23
5 DONG Bao Xia 1, WANG Zhe 2, LIANG Rong 2, CHEN Xie Qun 1, HUANG Gao Sheng 2, QIAO Yan 3, WANG Ai Qin 3 1Department of Hematology, Xijing Hospital, 2Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710033, China, 3Lanzhou Chemistry Physics Institute, Chinese Academy of Science, Lanzhou 730000, China;Inhibition of proliferation and induction of differentiation of leukemia cell line HL60 by glucosamine sulphate[J];Journal of The Fourth Military Medical University;2004-05
6 PEI Li,CHENG Jie ping,LIANG Hou jie (1 Department of Hematology;2.Department of Oncology,Southwest Hospital, the Third Military Medical Univercity,Chongqing 400038,China);Induction of dendritic cells differentiation from cord blood CD34~+ cells expanded ex vivo[J];Chongqing Medical Journal;2004-05
7 CHEN Jie-ping 1, Deborah Clarke 2, Constanze Bonifer 2 ( 1Department of Hematology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China; 2Molecular Medicine Unit, St. James's University Hospital, University of Leeds, Leeds LS9 7TF, UK);Roles of c-myb in haemopoietic differentiation and commitment of embryonic stem cells in vitro[J];Acta Academiae Medicinae Militaris Tertiae;2005-06
8 CHEN Long1, CHEN Jie-Ping1, LIN Chen2 1Department of Hematology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China, 2National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021, China;Effects of recombinant adenovirus encoding antisense c-myc on proliferation and cell cycle of HL-60 cells[J];Journal of The Fourth Military Medical University;2005-15
9 Qin Dabing, Chen Jieping, Wang Shenqi. Department of Hematology, Southwest Hospital, Third Military Medcial University, ChongQing 400038, China;Studies with an oligonucleotide microarray on the changes of gene expression related with NB4 apoptosis induced by arsenic trioxide[J];Medical Journal of Chinese People's Liberation Army;2007-05
10 YUE Baohong1),ZHU Xiaoyan2),WANG Qingxia1),LIU Shuai1),ZHANG Gongyuan2),ZHANG Qiutang3),ZHANG Hui2),ZHANG Qinxian2)1)Department of Clinical Laboratory,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)Department of Histology and Embryology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 3)Department of Hematology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;Effects of nucleostemin gene silencing on differentiation character of HL-60 cells in vitro[J];Journal of Zhengzhou University(Medical Sciences);2007-04
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 YUE Baohong1),ZHU Xiaoyan2),WANG Qingxia1),LIU Shuai1),ZHANG Gongyuan2),ZHANG Qiutang3),ZHANG Hui2),ZHANG Qinxian2)1)Department of Clinical Laboratory,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)Department of Histology and Embryology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 3)Department of Hematology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;Effects of nucleostemin gene silencing on differentiation character of HL-60 cells in vitro[J];Journal of Zhengzhou University(Medical Sciences);2007-04
2 SUN Yonggang,Department of Gastroenterology, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China;Advances in the research on nucleostemin[J];Chinese Journal of Gastroenterology and Hepatology;2008-01
3 SUN Yonggang,ZHOU Gang,WANG Weiqiang,YANG Shiming,WANG Rongquan,FANG DianchunDepartment of Gastroenterology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China;Study on nucleostemin expression in esophageal adenocarcinoma[J];Chinese Journal of Gastroenterology and Hepatology;2009-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved