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《Acta Academiae Medicinae Militaris Tertiae》 2005-21
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Effects of long-term morphine on the expression of fosB gene in in vitro cultured SK-N-SH cells

SU Yan-jun, ZHANG Guo-zhong, CONG Bin, ZHANG Jin, LI Shu-jin, YAO Yu-xia, FU Li-hong (Department of Forensic Science, Hebei Medical University, Shijiazhuang 050017, China)  
Objective To explore the effects of long-term morphine on the expression of fosB gene in cultured SK-N-SH cells. Methods Morphine-dependent SK-N-SH cells were established by culturing SK-N-SH cells with morphine for 48 h, then divided into morphine-dependent group and naloxone-withdrawn group in which the morphine-dependent SK-N-SH cells were treated with 10 μmol/L naloxone for 15 min. The SK-N-SH cells cultured with physiological saline served as normal control group. The intracellular cAMP, the DNA-binding activity of CREB, and the expression of fosB mRNA were detected by radioimmunoassay (RIA), electrophoresis mobility shift assay (EMSA), and RT-PCR respectively. Results The intracellular cAMP, the DNA-binding activity of CREB, and fosB mRNA were significantly elevated in morphine-dependent and naloxone-withdrawn SK-N-SH cells. Conclusion The upregulation of fosB mRNA in SK-N-SH cells treated with long-term morphine may be mediated through the activation of the DNA-binding activity of CREB.
【Fund】: 河北省卫生厅跟踪项目(2002-320)~~
【CateGory Index】: R749.6
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