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《Acta Academiae Medicinae Militaris Tertiae》 2006-03
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Deproteinized bone with VEGF gene transfer for avascular necrosis of the femoral head

CAO Kai~1,HUANG Wei~1,AN Hong~1,JIANG Dian-ming~1,HUANG Lu~2 (~1Department of Orthopaedics,The First Affiliated Hospital,~2Department of Renal Diseases,The Children's Hospital,Chongqing University of Medical Sciences,Chongqing 400016,China)  
Objective To explore a new method for early avascular necrosis of the femoral head(AVNFH) therapy.Methods Sixty-nine New Zealand adult rabbits with a mean weight of 2.8 kg after AVNFH establishment were randomly divided into 3 groups.In group 1,the deproteinized bone(DPB) combined with the recombinant plasmid pcDNA3.1/VEGF165 was implanted in the drilled channel of the necrotic femoral head.In group 2,only DPB was implanted.In group 3,only channel was drilled without DPB or plasmid implantation.Femoral head specimens were obtained at 3 d,1,2,4 and 8 weeks after operation. The expression of vascular endothelial growth factor(VEGF) and the security of this manipulation were examined by RT-PCR,Western blotting and immunohistochemical techniques.X-ray examined bone formation generally.Angiogenesis and repair of the femoral head were observed by histological and histomorphometric analysis.Results In group 1,the expression of VEGF was detected in the femoral head implanted by DPB-VEGF compound.The expression of VEGF165 mRNA and protein reached the maximum in 1 week after implantation and lasted more than 3 weeks,but none of them were detected in distant organs.The femoral head implanted by DPB-VEGF compound showed a significant difference in vascularization at 2 and 4 weeks and a significant difference in bone formation at 2,4 and 8 weeks after operation from those in other groups on histomorphometric analysis(P0.01).Conclusion The transfection of the VEGF165 gene enhances local angiogenesis in early stage and DPB-VEGF compound improves bone formation.VEGF gene therapy on rabbit AVNFH is effective and secure.The deproteinized bone combined with VEGF gene provides a potential method for therapy of osteonecrosis.
【Fund】: 重庆市卫生局资助项目(002010)~~
【CateGory Index】: R681.8
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