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《Acta Academiae Medicinae Militaris Tertiae》 2006-16
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Study on injury of human vascular endothelial cells by cholesterol

LU Zhi-shun,YU Xiao,TANG Jun-li,QIAN Min-zhang (Department of Biochemistry,Zunyi Medical College,Zunyi 563003,China)  
Objective To investigate the injuries caused by cholesterol to the vascular endothelial cells (VECs). Methods Different dosage of cholesterol (6.25,12.5,25.0,50.0 mg/L) was used on human umbilical endothelial cell line,ECV304,respectively. LDH activity,nitric oxide and the nitric oxide synthetase activity in the supernatant of cell culture were detected. The concentration of MCP-1 protein in cell culture was detected by ELISA. Results As compared with the normal control cells,a significant increase of LDH activity was found in the cells treated with 50.0 mg/L cholesterol. The NO level decreased in the cells treated by 25.0 or 50.0 mg/L cholesterol. When treated by cholesterol at dose of 6.25,12.5,25.0 or 50.0 mg/L respectively,the NOS activity was greatly decreased and MCP-1 protein was significantly increased in a dose-dependent manner. Conclusion Cholesterol of high concentration could directly injure the structure and partial function of VECs.
【Fund】: 贵州省科技厅基金资助项目(2003-3015)~~
【CateGory Index】: R363
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