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《Acta Academiae Medicinae Militaris Tertiae》 2006-19
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Inhibitive effect of cholecystokinin octapeptide on iNOS expression induced by lipopolysaccharide in ECV-304

GAO Feng,GU Zhen-yong,PING Jing,LIU Xia,ZHAO Li,XU Jin-rong,NI Zhi-yu,CONG Bin,LING Yi-ling((Department) of Forensic Medicine, Heibei Medical University,Shijiazhuang 050017,China)  
Objective To elucidate the molecular mechanism of cholecystokinin octapeptide(CCK-8) inhibiting of iNOS expression in ECV-304 induced by LPS.Methods ECV-304 cells,were stimulated with LPS in the presence or absence of CCK-8(10~(-9)-10~(-7)mol/L) or/and CCK receptor antagonist proglumide.The iNOS protein level in the cytoplasm at 16 h after stimulation was detected with Western blotting.The expressions of CCK-AR and CCK-BR mRNA were assayed with RT-PCR.The level of NF-κB P65 protein was detected in cytoplasm and nuclus at 1 h after stimulation with immunocytochemical technique.Results LPS led to the up-regulation of iNOS protein expression in ECV-304 that was obviously inhibited by CCK-8 in a dose-dependent manner.CCK-8 alone did not affect iNOS protein expression.CCK-AR mRNA expression was higher than CCK-BR mRNA expression in ECV-304.The expression of CCK-AR and CCK-BR mRNA could be up-regulated obviously by LPS.LPS increased NF-κB P65 protein expression in the nuclus of ECV-304.CCK-8 obviously inhibited LPS-induced changes of NF-κB P65 protein in a dose-dependent manner.The inhibitive effects of CCK-8 on iNOS protein expression and NF-κB P65 were attenuated by proglumide.Conclusion There were CCK-AR and CCK-BR mRNA expressions in ECV-304.CCK-8 inhibited LPS-induced up-regulation of iNOS protein expression by acting CCK receptors and NF-κB in ECV-304.
【Fund】: 教育部科学技术研究资助重点项目(204016);; 河北省自然科学基金资助项目(301351)~~
【CateGory Index】: R363
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