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《Acta Academiae Medicinae Militaris Tertiae》 2006-22
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Reconstruction of engineered bacteria in preparing recombinant human peptide antibiotic β

ZHANG Chun, CONG Yan-guang, HU Fu-quan, RAO Xian-cai (Department of Microbiology, College of Medicine, Third Military Medical University, Chongqing 400038, China)  
Objective To reconstruct the new engineered bacteria expressing hPAB-β triploids so as to improve the outputs of recombinant human peptide antibiotic β. Methods The recombinant plasmid pQE31-hPAB-β(3) was transformed into E. coli. M15 to screen the new engineered bacteria expressing hPAB-β triploids. The stabilities of phPAB-β(3)/M15 were observed in continuous cultures. The expression levels of the fusion peptides of interest and the bacterial yields of the new engineered bacteria phPAB-β(3)/M15 were compared with that of phPAB-β(3)/JM109 in different fermentation scales. Results Genetic stability of the recombinant plasmid and phPAB-β(3)/M15 was 100 after 10 passages. Take bacterial yields into account, the new engineered bacteria phPAB-β(3)/M15 was better than phPAB-β(3)/JM109 at the similar expression levels of the target proteins by “t” test analysis (P0.01). The yields of fermentation reached 55.15 g/L in 10 L fermenter, and the expression levels were up to 30.2 at 5th h after induction. During the fermentation, the recombinant plasmids were stable in culture. Conclusion The new engineered bacterium phPAB-β(3)/M15 successfully constructed was more consummate and effective on the basis of the original process.
【Fund】: 国家自然科学基金资助项目(30171119);; 全军医学科学技术研究“十五”计划基金重点课题(01Z070);; 国家“863”项目(2002AA214211)~~
【CateGory Index】: Q789
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1 MENG Jie-Ru, YAN Zhen, ZHAO Ning, ZHANG Ying-QiBiotechnology Centre, Department of Pharmaceutics, Fourth Military Medical University, Xi’an 710033, China;High cell density fermentation of engneering E. coli expressing tumor targeted interferon-α2a[J];Journal of The Fourth Military Medical University;2004-23
2 HU Jin-chuan, RAO Xian-cai, LI Shu et al. Department of Microbiology, Third Military Medical University, Chongqing 400038, China;Construction of recombinant plasmid harboring human peptide antibiotic gene hPAB-β and its expression in E. coli[J];Medical Journal of Chinese People's Liberation Army;2004-02
Chinese Journal Full-text Database 10 Hits
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2 WANG Sheng-xing et al (College of Modern Sciences and Technology,China Jiliang University,Hangzhou,Zhejiang 310018);Exploration on Preparation and Regeneration Conditions for Protoplast of Trichoderma viride[J];Journal of Anhui Agricultural Sciences;2010-08
3 HU Pei-Zhen1,LU Fan2,WANG Xiao-Gong2,LI Xia1,SI Shao-Yan1,HUANG Yang1,MA Bin1,ZHANG Xiu-Min1,SUI Yan-Fang11Department of Pathology, Xijing Hospital, 2Department of MolecularBiology and Biochemistry, School of Basic Medicine, Fourth Military Medical University,Xi’an 710033, China;High cell-density fermentation of engineered E.coli and antitumor immunity of recombinant human MAGE3/HSP70 in vivo[J];Journal of the Fourth Military Medical University;2007-03
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6 WANG Qi1,LIU Zuo-yi2*(1.Agricultural College,Guizhou University,Guiyang,Guizhou 550025;2.Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,China);Study on Liquid Fermentation Condition for Improving Yield of Paecilomyses cicadae Polysaccharide[J];Guizhou Agricultural Sciences;2010-06
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8 XIE Tao1,2,LIAO An-ping2,HUANG Chun-yu2,HOU Qiu-ping2(1.Inst.of Chemistry and Chemical Engineering of Guangxi University,Nanning 530004,China;2.Dept.of Chemistry and Ecoengineering of Guangxi University for Nationalities,Nanning 530006,China);Quick Measurement of Biomass in the System of Microbial Fermentation to Produce Mannitol by Nephelometery[J];Journal of Guangxi University for Nationalities(Natural Science Edition);2008-03
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10 WANG Du-liu,YE Wen-bin,YANG Jian-dong,HE Jiu-jun(Department of Biology&Chemistry,Longnan Teachers College,Chengxian Gansu 742500);The Brewing Process of Wheat-poured Wine[J];Journal of Gansu Normal Colleges;2012-05
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8 BAI Xue-jing1,XIONG Xiao-chao 2,JIANG Sheng-hua 1,LIU Hui-zhou 2,LI Xin 1(1.Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China;2.Key Lab.Green Process Eng.,Inst.Process Eng.,Chinese Academy of Sciences,Beijing 100080,China);Study on Specific Desulfurization Activity and Related Genes of Rhodococcus sp.LY822[J];The Chinese Journal of Process Engineering;2008-01
9 YANG Ling-chao~1,SUN Ai-you~1,SHEN Ya-ling~1,WEI Dong-zhi~1,GONG Yi~2,MA Yu-shu~1(1.State Key Laboratory of Bioreactor Engineering,Institute of Biochemistry,East China University of Science and Technology,Shanghai 200237,China;2.Shanghai Research Center of Biotechnology,Shanghai Institutes for Biological Science,Chinese Academy of Science,Shanghai 200233,China);Cloning and Expression of Vitreoscilla Hemoglobin in Escherichia coli for TRAIL Production[J];Journal of East China University of Science and Technology(Natural Science Edition);2006-08
10 WU Di,YANG Mingming,ZHANG Yani,et al(1.College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100 , China;2.YangGuang-GuangJi Medical R&D co. Lt, WuHan 430062, China);The construction and inducing expression of veb expression vector[J];Heilongjiang Journal of Animal Science and Veterinary Medicine;2005-06
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 Sun Lequn;Ge Jingping;Wang Lili;Ping Wenxiang;Key laboratory of microbiology of Heilongjiang province/College of Life Science, Heilongjiang University;;The Optimal Desulfurization Conditions of Desulfurization Strain with High Dibenzothiophene Degradation Ability[J];Chinese Agricultural Science Bulletin;2013-30
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 RAO Xian cai, JIN Xiao ling, HU Xiao mei, ZHU Jun ming, CHEN Zi jin,HU Fu quan (Department of Microbiology, Third Military Medical University, Chongqing 400038,China);Design of a carrier molecule and high level expression of peptide antibiotics hPAB-β[J];Acta Academiae Medicine Militaris Tertiae;2002-04
2 MENG Jieru,YAN Zhen,ZHAO Ning et al (Biotechnology Center, The Fourth Military Medical University, Xi'an 710032);Stability of Biological Characteristics of Recombinant Bacterial Strain Ex-Tumor Targeted IFNα2a[J];Chinese Journal of Biologicals;2003-04
3 RAO,Xian cai,HU Xiao mei,ZHANG Ke bin,et al.(Department of Microbiology,Third Military Medical University,Chongqing 400038);Cloning and expression of human β-defensin-2 in E.coli[J];Progress In Microbiology and Immunology;2001-03
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