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《Acta Academiae Medicinae Militaris Tertiae》 2007-14
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On HBV infection of trophoblastic cells in primary culture

LI Jun-nan1,WANG Yu-ming2,SHI Chang-xu1,LIANG Zhi-qing1,CHANG Qing1,ZHANG Qin1,CHEN Li1(1Department of Obstetrics and Gynecology,2Institute of Infectious Diseases,Southwest Hospital,Third Military Medical University,Chongqing 400038,China)  
Objective To investigate the possibility and regulation of that hepatitis B virus(HBV)infects trophoblastic cells in primary culture.Methods Trypsin and DNAse sequential digestion and Percoll density centrifugation were used to separate and purify human chorionic trophoblastic cells.The direct serum(HBV DNA positive)infection method was adopted to infect trophoblastic cells.The results of HBV infection of trophoblastic cells were compared with that of normal human adult liver cell line L02 and that of liver cancer cell line HepG2.2.15.Real-time PCR was used to detect HBV DNA in culture supernatant.Further,ultrastructural characteristics of infected trophoblastic cells were observed with transmission electron microscope.Results The HBV DNA in culture supernatant that released by HBV infected trophoblastic cells reached the peak in 48-72 h.For L02 cell,it occurred in 12-24 h and for HepG2.2.15 cell line,it kept increasing.The ultrastructure of infected HBV trophoblastic cells differed obviously from that of normal cells,and showed hyperplasia of lysosome,appearance of vacuoles and virus-like particles around the nucleus.Conclusion Trophoblastic cells could be infected by HBV,and the cellular ultrastructure changed dramatically following HBV infection.
【Fund】: 国家自然科学基金(30230320 30400479)~~
【CateGory Index】: R361
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