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《Acta Academiae Medicinae Militaris Tertiae》 2008-09
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Gender difference of impaired pancreatic function induced by long-term high-fat diet and its underlying mechanism

WANG Zhe1,GAN Li-xia1,LU Zhong-yan1,HE Feng-tian1,ZOU Quan-ming2(1Department of Biochemistry and Molecular Biology,College of Medicine,2Department of Clinical Microbiology,College of Laboratory Medicine,Third Military Medical University,Chongqing 400038,China)  
Objective To explore the effect of long-term high-fat diet(HF)on glucose tolerance and insulin action in male and female SD rats and the underlying mechanism.Methods Totally 100 SD rats were equally divided by gender and further subgrouped into standard laboratory chow(SC,n=20)and HF groups(n=30),respectively.Body weight and fasting glucose levels were measured every month,and intraperitoneal glucose tolerance test(IPGTT)was performed in the 5,8,10 and 14 months.At the 14th month,fasting plasma insulin concentration was measured,and the contents of malondialdehyde(MDA),glutathione(GSH),and catalase in different fraction of pancreatic homogenates,mitochondria or microsome,were determined.Results Impaired glucose tolerance was observed in both SC and HF male rats but not in female rats.Fasting insulin levels and homeostasis model assessment of insulin resistance(HOMA-IR)were higher in male rats than in female rats from SC groups and significantly higher in HF feeding rats.Whereas,insulin secretion capacity was significantly lower in male than in female.MDA in pancreatic mitochondria was higher and GSH and catalase in pancreatic cytosol was lower in male than in female.Conclusion Female are relatively protected from induced glucose tolerance under HF feeding and is likely,at least in part,from ameliorated oxidative stress in pancreas.
【Fund】: 国家自然科学基金(30570888 30670998);; 全军医学科研“十一五”计划国际合作项目(06H025)~~
【CateGory Index】: R587.1
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