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《Acta Academiae Medicinae Militaris Tertiae》 2008-18
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Growth inhibition effects of artesunate on human cervical cancer cell line HeLa

WANG Ming-hua1,ZHENG Jun-sheng2,LUO Yun-peng3,MI Can1(1Department of Pathology,3Department of Pathophysiology,Faculty of Basic Medical Sciences,Chongqing Medical University,Chongqing 400016;2Department of Gynaecology and Obstetrics,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)  
Objective To investigate the effects of artesunate on human cervical cancer cell line HeLa.Methods The inhibitory effect on cell proliferation was assessed by MTT assay.Transmission electron microscopy and fluorescent staining were applied to demonstrate the presence of apoptosis.Cell cycle,reactive oxygen species(ROS) levels and mitochondrial membrane potential(ΔΨm) were examined by flow cytometry,respectively.Expression of caspase-3 was detected by immunohistochemical methods.Results Growth inhibition of ART on HeLa cells was time-and dose-dependent.Apoptotic feature was observed by transmission electron microscopy and fluorescent staining.The blocking of the cell cycle was in S-phase.The expression of caspase-3 in cytoplasm was positive.The generation of ROS increased obviously(P0.01) after ART treated the cells for 72 h,while the levels of ΔΨm were decreased(P0.05).Conclusion Artesunate inhibits the proliferation of HeLa cells,which action may be related to increasing of the cellular ROS level and decreasing of mitochondrial member potential,resulting in cell apoptosis.
【CateGory Index】: R285.5;R737.33
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Chinese Journal Full-text Database 5 Hits
1 WANG Jing, LIU Liang, LI Jin-mei, LIU Jiang-hui, GUO Jian-wen, ZUO Lian-fu (Department of FCM Analysis, Hebei Provincial Tumor Institute, Fourth Hospital Affiliated to Hebei Medical University, Shijiazhuang 050011, China);Inhibitory effect of artesunate on human esophageal carcinoma is associated with CDC25A modulation[J];Acta Academiae Medicinae Militaris Tertiae;2007-05
2 ZHANG Yuchuan CHEN Shiyao ZHANG Youli Deparment of Gastroenterology, Jiangsu University. Jiangsu Zhenjiang 212001; Deparment of Gastroenterology, Zhongshan Hospital , Fudan University, Shanghai 200032;Effects of Artemisunate on Expression of Intercellular Adhesion Molecule-1 in Human Colon cancer Line HCT116[J];Chinese Journal of Clinical Medicine;2006-05
3 YUAN Chang Qing, DING Zhen Hua (Laboratory of Radiation Medicine, Department of Tropical Military Hygienics, The First Military Medical University, Guangzhou 510515);Activation and Function of Caspases in Apoptosis[J];Progress In Physiological Sciences;2002-03
4 ZHAO Jun-ning,HE Yi-ran,ZHANG Zhen-yu, et al (Depa rtment of Gastroenterology, The Official Hospital of Jiangsu Province, Nanjing Jiangsu 210024,China);Effects of artesunate on the proliferation and apoptosis of gastric carcinoma cells[J];China Oncology;2005-04
5 CHEN Shao-fang1,XU Yi-yi1,LIU Xiang-lin2,et al.(1.Tiantai People's Hospital,Tiantai 317200,China;2.School of Medicine,Zhejiang University,Hangzhou 310031,China);Inhibitive Effect of Artesunate on Heps Hepatona in Mice[J];Bulletin of Chinese Cancer;2004-06
Chinese Journal Full-text Database 10 Hits
1 MU Hong-sheng1,LI Xiao-mei2,QI Zhen-xiong3,LI Xu-e1 1.College of Life Sciences,South China Normal University,Guangzhou 510631,China;2.College of Life Sciences,Guangzhou University,Guangzhou 510006,China;3.Haida Research Center of Livestock Husbandry and Fisheries in Guangdong,Guangzhou 511400,China);Application of Chinese Herb Medicines in Therapeutic Treatments of Parasitic Fish Diseases[J];Fisheries Science;2008-11
2 XING Chun-yong,SUN Xiao-ai,GAO Min,ZHANG Wen-qin(Department of Chemistry,School of Science,Tianjin 300072,china);Simple Total Synthesis of 1-Carbethoxy-2-Methyl-4-Piperidone[J];Information Recording Materials;2009-01
3 KUANG Hua,ZHANG Ji-zhen,JIA Hong-bin,ZHAO De-jian,WANG Ya-zhen(School of Chemistry and Chemical Engineering,Jiangsu Teachers University of Technology,Changzhou 213001,China);Teaching Reform Attempt of Organic Chemistry[J];Journal of Jiangsu Teachers University of Technology;2007-04
4 LI Jin-mei,WANG Jing,LIU Jiang-hui,GUO Jian-wen,ZUO Lian-fu(Cytometry Laboratory,Tumor Institute,The Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China);Expressions of CDC25A and Smad3 in esophageal squamous cell carcinoma and their relationship[J];Acta Academiae Medicinae Militaris Tertiae;2008-03
5 LIU Xiao-yun1,XIONG Zheng-ai1,ZHENG Hong2(1Department of Obstetrics and Gynecology,Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010;2Department of Pathology,Affiliated Hospital,Zunyi Medical University,Zunyi,563003,Guizhou Province,China);Expression and significance of pro-apoptotic factors and anti-apoptotic factors in cervical intraepithelial neoplasia and cervical carcinoma[J];Acta Academiae Medicinae Militaris Tertiae;2009-17
6 QIN Ming-li,LI Hua-chun,TAN Ke-jun(Education Ministry Key Laboratory on Luminescence and Real-time Analysis,School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715);Determination of Paracetamol by Resonance Light Scattering Technique[J];Journal of Analytical Science;2011-06
7 LIU Jie,LIU Yan-mei and ZHANG Yong(School of Chemistry and Chemical Engineer,Shanxi University,Taiyuan 030006);Study on electrochemical behaviors and analytical application of doxorubicin hydrochloride at a glassy carbon electrode[J];Chinese Journal of Analysis Laboratory;2008-06
8 Xue Yuanying, Pan Meizhen, Gong yujun (College of Chemistry and Life Science, Maoming University, Maoming 525000, China);Determination of Lidocaine Hydrochloride by Flow Injection Chemiluminescence Method[J];Guangdong Chemical Industry;2009-08
9 LIU Yi-Ming GUO Jun-Jun MA Xiao XUAN Chun-Sheng a(Shanxi Analysing and Testing Center,Taiyuan 030006,P.R.China) a(Key Laboratory of Coal Science and Technology of Ministry of Education,Shanxi Province and Taiyuan University of Technology,Taiyuan 030024,P.R.China);Determination of Dissolution of Fosfomycin Sodium by Spectrophotometry with Alizarin[J];Chinese Journal of Spectroscopy Laboratory;2010-04
10 HUO Cai-xia,BAI Lin,REN Jian-jian(Department of Chemistry,Lanzhou City University,Lanzhou 730070,China);Interaction between Ceftazidime and Bovine Serum Albumin Found by Spectroscopy[J];Journal of Gansu Sciences;2008-04
Chinese Journal Full-text Database 10 Hits
1 Zhao Tao~1 Zhang Tingrong~1 Liu Jian~2 Shen Weisheng~1 1 Cancer Center of Jiangyin People's Hospital Affiliatedof Dongnan Unisersity,Jiangyin 214400 China 2 Tumor Hospital of Fujian Province,Fuzhou 350014 China;Influence of Yisheng Yin-contained serum on human lung cancer cell line A549[J];Oncology Progress;2008-03
2 Wan Xuying,Zhang Cheng,Ling Changquan,et al Changhai Hospital of Second Military Medical University,Shanghai(200433);Anti-hepatocarcinoma Activity and Immunopharmacological Effects of Maorenshen Injection[J];Journal of Zhejiang College of Tcm;2004-04
3 XU Guang-xing(HE Ren Institute of Traditional Chinese Medicine,Zhejiang University of Traditional Chinese medicine,Hangzhou 310053,China);Exploration on academic ideas of professor HE Ren in treating primary hepatic carcinoma[J];China Journal of Traditional Chinese Medicine and Pharmacy;2008-07
4 Ryoji Katoh1,2 and Mitsuru Ooshiro1 1Department of Surgery,Toho University Sakura Medical Center,Sakura-shi,Chiba,Japan;;Enhancement of Antitumor Effect of Tegafur/Uracil(UFT) plus Leucovorin by Combined Treatment with Protein-Bound Polysaccharide,PSK,in Mouse Models[J];中国免疫学杂志(英文版);2007-04
5 LIANG Ke ming, XIE Yan hua, SHI Mei, WANG Si wang, WANG Xiao juan (Radiotherapy Center, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China);Inhibitory effect of Ardipusillide on human cervical carcinoma cells[J];Acta Academiae Medicine Militaris Tertiae;2002-06
6 JIANG Zhong-yong,MEI Feng,YU Bin,HAN Juan,TIAN Yan-ping,LI Shi-feng,ZHOU De-shan(Department of Histo-logy and Embryology,College of Basic Medical Sciences,Third Military Medical University,Chongqing 400038,China);Artesunate inhibits proliferation and apoptosis of epidermal squamous cell line A431[J];Acta Academiae Medicinae Militaris Tertiae;2009-07
7 ZHAO Xin-Han, WANG Zhi-Yu, LI Jing, QUAN Ping Department of Oncology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi'an 710061, China;Bcl-2 antisense oligodeoxynucleotides to enhance the apoptosis-inducing effect of artesunate on K562 cells[J];Journal of the Fourth Military Medical University;2006-14
8 ZHENG Jun-sheng1, WANG Ming-hua2, HUANG Min1, LUO Yun-peng3, MI Can2 1Department of Gynecology and Obstetrics, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; 2Department of Pathology, 3Department of Pathophysiology, Chongqing Medical University, Chongqing 400016, China;Artesunate suppresses human endometrial carcinoma RL95-2 cell proliferation by inducing cell apoptosis[J];Journal of Southern Medical University;2008-12
9 HUANG Zeliang,ZHOU Xi,CHEN Zhongdong,et al. (The Second People′s Hospital of Huaihua,Huaihua 418400,China);Apoptosis and activity change of telomerase induced by paclitaxel in cervical cancer HeLa cells[J];Chinese Journal of Clinical Obstetric and Gynecology;2005-03
10 PENG Jun,LIN Jiu-mao,WEI Li-hui,et al(Fujian Academy of Integrative Medicine,FUTCM,Fuzhou,Fujian 350108,China);Mechanism of Apoptosis of Human Colon Cancer HT-29 Cells Induced by Scutellaria barbata D.Don Extract[J];Journal of Fujian University of Traditional Chinese Medicine;2010-06
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 YANG Yan;ZHANG Xiaomin;WANG Xiaofen;ZHAO Xiaomei;YU Bo;REN Tianrui;WANG Feng;College of Life and Environment Sciences,Shanghai Normal University;Pushikang Biological Technology Co.,Ltd;;Proliferation inhibition study of artesunate-transferrin adduct on cancer cells[J];Journal of Shanghai Normal University(Natural Sciences);2013-06
2 CHEN Jin-luan;WANG Cui-zhu;LI Ping-ya;HAN Hong-xiang;College of Pharmacy,Jilin University;Jilin Agricultural University;Jilin Institute of Biology;;Research Progress of Chemical Constituents and Pharmacological Effects of Patrinia Juss[J];Special Wild Economic Animal and Plant Research;2014-03
3 CHI Ai-ping;KANG Chen-zhe;GUO Huan-huan;LI Hong;WANG Yi-min;GUO Fei;School of Sports, Shaanxi Normal University;;Composition of Polysaccharides from Herba Patriniae and Their Anti-fatigue and Anti-hypoxia Activities[J];Food Science;2014-21
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Zhou Ping; Gao Yuxiang(Department of Dermatology,Affiliated Hospital of Bengbu Medical College,Anhui 233004);Effects of artesunate on immune functions in mice[J];ACTA ACADEMIAE MEDICINAE BENGBU;1996-01
2 WANG KAI-HUA (Shandong Normal University);A IN VITRO CELL LINE (MGc80-3) OF A POORLY DIFFERENTIATED MUCOID ADENOCARCINOMA OF HUMAN STOMACH[J];Acta Biologiae Experimentalis Sinica;1983-03
3 XIAO Liu ying,LIN Pei yin,ZHANG Dan,et al. Guangzhou Institute of Traditional Chinese and Materia Madica,Guangzhou,510130;Inhibition Effects of Artesunate on Hepatic Carcinoma and S_(180) Solid Tumor in Mice[J];The Practical Journal of Cancer;2001-03
4 LIN Fang1, QIAN Zhi-yu1, XUE Hong-wei2, DING Jian3, LIN Li-ping3 (1.Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China; 2. National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, CAS, Shanghai 200032, China; 3. Shanghai Institute of Materia Medica, CAS, Shanghai 200032, China);Comparison of inhibitory effects between artemisinin and artemisunate on proliferation of MCF-7 cells in vitro[J];Chinese Traditional and Herbal Drugs;2003-04
5 LIN Pei-Ying, FENG Zhao-Ming, PAN Jing-Qiang, ZHANG Dan, XIAO Liu-Ying (Department of Pharmacology, Guangzhou Institute of Medicine and Health, Guangzhou 510180, China);Effects of artesunate on immune function in mice[J];中国药理学报(英文版);1995-05
6 WANG Qin, WU Li mao, LI Ai yuan, ZHAO Yi, WANG Nai ping (Guangxi Traditional Chinese Medical University, Nanning 530001, Guangxi, China);Experimental Studies of Antitumor Effect of Artesunate on Liver Cancer[J];China Journal of Chinese Materia Medica;2001-10
7 Chen Zheagtu, Huang Zhenyan , Wu Lingni. et al. Tropical Medicine Institute, Guangzhou University of Traditional Chinese Medicine (Guangzhou 510405);Experimental Investigation of Apoptosis of Hepatoma Mediated by Artemisinin[J];Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases;2000-05
8 WU Li mao,ZHAO Yi,WANG Qin,et al;Preliminary Mechanism of Artesunate Treating Hepatocarcinoma[J];China Journal of Basic Medicine In Traditional Chinese Medicine;2002-08
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