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《Acta Academiae Medicinae Militaris Tertiae》 2008-21
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Multilineage differentiation of murine embryonic mesenchymal stem cell line C3H/10T1/2

WANG Ming-ke,ZOU Zhong-min,SU Yong-ping,LUO Cheng-ji,WANG Jun-ping,RAN Xin-ze,WANG Tao,YAN Guo-he(State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Combined Injury,College of Preventive Medicine,Third Military Medical University,Chongqing 400038,China)  
Objective To establish experimental cell models of myogenic cells,adipocytes,endothelial cells and neurocytes differentiated from murine embryonic mesenchymal stem cell line C3H/10T1/2.Methods Myogenic and adipogenic differentiation of C3H/10T1/2 cells were induced by 5-azacytidine,angiogenic differentiation by vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF),and neurogenic differentiation by bFGF,β-mercaptoethanol(β-ME) and DMSO.The differentiated cells were identified by observing cell morphology,Oil Red O staining and immunohistochemical staining to detect endothelial cell surface marker CD31 and neuron-specific enolase(NSE).Results C3H/10T1/2 cells were morphologically elongated at the 10th day,and myotube-like cells appeared at the 17th day after 5-azacytidine induction.Meanwhile,adipose globelets were observed in a small proportion of cells at the 17th day,and Oil Red O staining confirmed adipose accumulation in the cytoplasm at the 25th day after 5-azacytidine induction.Cobble stone-like structure was observed since 5th day after inducement of VEGF and bFGF,and at the 8th day the induced cells exhibited typical CD31 positive staining.After treated with bFGF,β-ME in combination with DMSO,cytoplasm retracted towards the nucleus and the cell processes prolonged at the 3th day.Immunohistochemical staining showed that differentiated cells expressed NSE at the 15th day.Conclusion C3H/10T1/2 cells have the potential of multilineage differentiation and can be used as an alternative source of mesenchymal stem cells.Growth factor-induced endothelial differentiation of C3H/10T1/2 cells was established.
【Fund】: 国家重点基础研究发展计划(973计划)(2005CB522605);; 全军医学科研“十一五”计划国际合作项目(06H029 06H030)~~
【CateGory Index】: R-33
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