Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicinae Militaris Tertiae》 2009-05
Add to Favorite Get Latest Update

Establishment of classical eyeblink conditioned reflex model in conscious and unfixed guinea pigs and study of related acquisition rule

LI Rui1,LIU Hong-liang1,SUI Jian-feng2,FAN Zheng-li2,HUANG Lu-shuai2,WAN Zi-bing3,ZHAO Hong-mei3(1Department of Rehabilitation and Physical Therapy,Southwest Hospital;2Department of Physiology,3Experimental Center of Experimental Teaching,College of Basic Medical Sciences,Third Military Medical University,Chongqing 400038,China)  
Objective To establish a model of classical eyeblink conditioned reflex(EBCR) in conscious and unfixed guinea pigs and to analyze the related acquisition rule.MethodsWith the aid of metal electrode embedding technique and simple follow-up air puff stimulus facility,conscious and unfixed guinea pigs were trained to performed EBCR with tone as conditioned stimulus(CS) and air puff as unconditioned stimulus(US).The eyeblink behavior was evaluated through electromyography(EMG) of musculi oculi.ResultsThe eyeblink EMG signal of musculi oculi were successfully collected,and the classical eyeblink conditioning was obtained.The acquisition rate in the 4th,7th,and 10th day were 31%,61.3% and 82.7% respectively,and the maintain rate in the 15th and 20th day were 81.3% and 82.3%.ConclusionConscious and unfixed guinea pigs can be trained to obtain EBCR with the techniques of musculi oculi EMG signal recording and follow-up air puff stimulating method.It is an easy,convenient,feasible,and reliable method with high achievement ratio.It could be applied to study conditioned eyeblink behavior of animals with big body type,or animals uneasy to be fixed.
【Fund】: 国家自然科学基金(30672255)~~
【CateGory Index】: R-332
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 ;An automatic training system suitable for low-grade mammals in visual and auditory information discrimination tests[J];Acta Academiae Medicinae Militaris Tertiae;2005-01
Chinese Journal Full-text Database 10 Hits
1 Jia Huijuan1 Li Yuan1 Wu Juanjuan1 Zhang Anmin2(1.School of Phydical Education,Shanxi University,Taiyuan Shanxi 030006;2.School of Physical Education,Shanxi University of Finance and Economics,Taiyuan Shanxi 030006);Exercise and the Hippocampal cAMP Research[J];Sport Forum;2010-01
2 YUAN Guo hong,GAO Wei juan,MENG Fan xing,et al (Department of Pathophysiology,Chengde Medical College,Hebei Chengde,067000,China);EFFECT OF YISHENJIANGZHUO DECOCTION ON INTRACEREBRAL ACHE ACTIVITY OF ISCHEMIA REPERFUSION RAT[J];Journal of Chengde Medical College;2001-02
3 GAO Jie, WU Yamin. State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China;Research progress on traumatic memory in posttraumatic stress disorder[J];Chinese Journal of Neuromedicine;2005-01
4 QIAN Ruo-bing FU Xian-ming NIU Chao-shi WANG Ye-han WEI Jian-jun LU Ming WANG Lin.Department of Neurosurgery,Anhui Provincial Hospital Affiliated to Anhui Medical University,Hefei 230001,China;Correlation between learning and memory impairment and the changes of intracellular free Ca~(2+) concentration in hippocampal neurons after temporal lobe epilepsy in rats[J];Chinese Journal of Neuromedicine;2007-11
5 YU Ruo-gu,LI Gang,ZHONG Xiao-yun,et al.(Chongqing Maternal and Child Health Institute,Chongqing 400013,China);Study of effect of enriched environmental inteference on neurons in hippocampus of HIBD neonatal rats[J];Chongqing Medicine;2009-22
6 LU Cheng-Qiu, ZHONG Le, TIAN Ying, YAN Chong-Huai, SHEN Xiao-Ming. Shanghai Key Laboratory of Children's Environmental Health,Shanghai Institute for Pediatric Research,Shanghai Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China;Effects of preweaning enrichment on activity-regulated cytoskeletal protein expression and long-term memory in rats[J];Chinese Journal of Contemporary Pediatrics;2008-02
7 LIN Xi1, HU Bo2, YANG Li1, SUI Jian-feng1 (1 Department of Physiology, College of Medicine; 2 Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Microinjection of Muscimol into cerebellular dentate-interpositus nuclei affects acquisition of classical eyeblink conditioning in guinea pigs[J];Acta Academiae Medicinae Militaris Tertiae;2008-06
8 HUANG Lu-shuai1, LIN Xi1, HU Bo2, LI Rui3, YANG Li1, SUI Jian-feng1 (1Department of Physiology, College of Basic Medical sciences, 2Department of Neurosurgery, 3Department of Rehabilitation, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Synaptic ultrastructural changes of cerebellar dentate-interpositus nuclei after acquisition of classically conditioned eyeblink responses in guinea pigs[J];Acta Academiae Medicinae Militaris Tertiae;2009-06
9 ZHANG Feng1, YAO Hong-bing1, LI Bing2, LI Min3 (1Department of Otorhinolaryngology, Children’s Hospital, Chongqing Medical University, Chongqing 400016, 2Department of Otorhinolaryngology, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400014; 3Department of Psychology, College of Nursing, Third Military Medical University, Chongqing 400038, China);Effects of chronic intermittent hypoxia on learning and memory ability and GFAP expression in CA3 region of hippocampus in rats[J];Acta Academiae Medicinae Militaris Tertiae;2009-12
10 YANG Li,HU Bo,FAN Zheng-li,HUANG Lu-shuai,CHEN Hao,SUI Jian-feng ( Department of Physiology,College of Basic Medicine Sciences,Third Military Medical University,Chongqing 400038,China);Effects of different recording methods for orbicularis oculi muscle activity on acquisition of trace eyeblink conditioning in guinea pigs[J];Acta Academiae Medicinae Militaris Tertiae;2009-18
China Proceedings of conference Full-text Database 3 Hits
1 ;The difficulty judgment of Chinese-English source material for interpreting[A];[C];2011
2 ZHANG Ri-hui LIU Yu HAN Zhong-yuan (Department of Sports Physiology and Biochemistry,Shenyang Sport University,Shenyang 110102);Influence of Fatigue by Training on Memory and Hippocampus Glutamate in the Rat[A];[C];2007
3 LI Hao,YAO Ming-jiang~*,XU Li,LIU Jian-gang,ZHAO Wen-ming (Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091 China);Effects of Huannao Yicong Decoction on Cognitive Function and Expression of Bcl-2 and Bax Protein of Hippocampus in Cognitive Impairment Rats[A];[C];2009
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved