Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicinae Militaris Tertiae》 2009-09
Add to Favorite Get Latest Update

Establishment of in vitro oxygen-glucose deprivation/reoxygenation axon injury model in rat hippocampal neurons

YIN Cheng, QIN Xin-yue, WU Xiao-hui (Department of Neurology, Key Laboratory of Neurology of Chongqing, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China)  
Objective To establish an in vitro oxygen-glucose deprivation/reoxygenation axon injury model in rat hippocampal neurons in order to found a basis for research concerning the injury of oxygen glucose deprivation and axons regeneration. MethodsThe hippocampal neurons isolated from rats 24 h after born and cultured for 7 d were exposed to D-hanks solution with nitrogen gas instead of the original culture medium for 0.5, 1.0 and 1.5 h respectively, and then continue to be cultured in the original culture medium with oxygen. LDH content in the culture media was measured at 1, 5, 24, 48 and 72 h after reoxygenation. The morphological changes of neuron and axon were observed with inverted phase contrast microscopy. ResultsAfter oxygen-glucose deprivation/reoxygenation injury, hippocampal neurons became darker, swollen, and were with shorten axons. With the elapse of time, the LDH content was increased. The survival rate of hippocampal neurons was higher and the change of axon length was more obvious in the group of oxygen-glucose deprivation for 0.5 h than in the other groups. ConclusionAn in vitro oxygen-glucose deprivation/reoxygenation axon injury model in rat hippocampal neurons is successfully established.
【Fund】: 国家自然科学基金(30770762)~~
【CateGory Index】: R-332
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 5 Hits
1 LIU Zhiliang, XU Ruxiang Department of Neurosurgery, Neuromedical Institute of PLA, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China;Protective effect of inhibition of p38 MAPK signal transduction on toxicity damage to hippocampal neurons[J];Chinese Journal of Neuromedicine;2003-03
2 XU Zhong*, XU Ru-xiang*, LIU Bao-song, HUANG Tao*, DING Lian-shu*, YUAN Jun*. *Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China;Changes of GluR2 on post-synaptic membrane of rat hippocampal neurons induced by oxygen and glucose deprivation[J];Chinese Journal of Neuromedicine;2005-03
3 GAO Yuan-jun, QIAN Wei, WANG Bu-hai, LIN Rong, HOU Xiao-hua (Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China);Neural differentiation and neurotrophic factors expression in rat bone marrow stromal cell[J];Acta Academiae Medicinae Militaris Tertiae;2006-21
4 WEN Can1, LI Shu-rong2, ZHANG Yan-ling1, WANG Han-zhi1, SU Bing-yin1, 3 (1Department of Neurobiology, College of Medicine, Third Military Medical University, Chongqing 400038;2Department of Pathology,3Department of Histology and Embryology and Neurobiology, Chengdu Medical College, Chengdu 610081, China);Effects of GDNF and substrate on superior cervical ganglion sympathetic neurons in vitro[J];Acta Academiae Medicinae Militaris Tertiae;2007-10
5 WAN Hai-tong WANG Yu YANG Jie-hong Institute of Cardio cerebrovascular Disease,Zhejiang Chinese Medical University,Hangzhou (310053);Establishment of Oxygen and Glucose Deprive Model of in vitro Cultured Hippocampal Neuron and Effect of Ligustrazine on Intracellular Ca~(2+) Level in Model Neurons[J];Chinese Journal of Integrated Traditional and Western Medicine;2007-03
Chinese Journal Full-text Database 6 Hits
1 XIA Yuan LI Wen-jun ZOU Fei. School of Public Health and Tropical Medicine,Southern Medical University,Guangzhou 510515,China;The study of hypoxia tolerance of hippocampal neurons from 5-day-old rats[J];Chinese Journal of Neuromedicine;2007-08
2 Zhou Junsong,Dong Yunhai,Song Xuehui,Xin Lian,Zhang Huanxiang(Medical College of Soochow University,Jiangsu Province Key Laboratory of Stem Cell Research,Suzhou 215123,China);Proliferation of neuronal precursor cells in serum-free culture[J];Chinese Journal of Anatomy;2008-06
3 MEI Li1,2,LU Hao1,LIU Zong-ping1,HE Yu-qin2(1.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China);Primary culture and viability assessment of the cerebral cortical neurons from newborn SD rat[J];Veterinary Science in China;2007-03
4 YANG Rui PENG Rui-yun GAO Ya-bing WANG Shui-ming HU Wen-hua XU Xin-ping SU Zhen-tao HU Xiang-jun XU Tian-hao.Beijing Institute of Radiation Medicine.Beijing 100850,China;The effect of microwaves on hippocampal neurons in vitro and its mechanism[J];Chinese Journal of Physical Medicine and Rehabilitation;2006-10
5 QI Yue-ru,PAN Yan-shu.(Department of Pathology,College of Basic Medicine,Beijing University of Chinese Medicine,Beijing 100029,China);Advancements in the Research of the Changes of Ions in Cerebrospinal Fluid in Cerebral Ischemia[J];Medical Recapitulate;2009-17
6 HU Ya-Zhuo,WANG Xue-Li,Lü Pei-Yuan.Department of Neurology, Hebei Province People's Hospital,Shijiazhuang 050051,Hebei,China;The expression of p38MAPK mRNA in neuron of hippocampus in mice with vascular dementia[J];Chinese Journal of Gerontology;2009-23
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 LIU Bao song,LONG Zai yun,ZENG Lin,et al. (Research Institute of Surgery,Daping Hospital,Third Military Medical University,Chongqing 400042,China);Experimental study on the changes of GluR2 on post-synaptic membrane and its related brain secondary injury[J];Journal of Traumatic Surgery;2003-06
2 ZHANG Wen-jie 1, ZHOU Yue 1, CHEN Jing 2, WANG Jian-zhong 1, CHEN Jian-mei 2 ( 1Department of Orthopaedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China);Effects of GDNF-modified Schwann cells on repair of sciatic nerve defect in rats[J];Acta Academiae Medicinae Militaris Tertiae;2004-20
3 WANG Yonggang 1, 2) , ZHANG Yong 1) ,ZHANG Qi 1) , WU Aiqun 3) , HE Cheng 1) 1)Department of Neurobiology, the Second Military Medical University, Shanghai 200433 2)Department of Human Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052 3)Department of Anatomy, the Second Military Medical University, Shanghai 200433;Expression and distribution of nerve growth factor receptor TrkA in rat spinal cord[J];Journal of Zhengzhou University(Science Medical);2004-03
4 WANG Ying, CHE Yu, MIAO Jian ting, LI Zhu yi, WANG Zhe jin PLA Center of Neurology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China;The study of primary culture methods for hippocampal neurons of newborn rats[J];Journal of Cellular and Molecular Immunology;2003-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved