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《Acta Academiae Medicinae Militaris Tertiae》 2009-15
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The apoptotic pathway of breast cancer multidrug resistant cells

ZHANG Xue-mei1,YANG Xiu-rong1,WU Hua2,ZHANG Zhen-wei2,XIANYU Zhi-qun2(1Department of Nuclear Medicine,Wuhan General Hospital of Guangzhou Military Command,Wuhan 430070;2Department of Nuclear Medicine,Tongji Hospital Subordinated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)  
Objective To investigate the possible apoptotic pathway in breast cancer daunorubicin-resistant subline,MCF-7/Adr cell line.Methods Drug-resistance cell line MCF-7/Adr expressing functional P-gp and its wild type MCF-7/wt cell line were used for comparison.The concentration of hydrogen peroxide(H2O2)that may lead to loss of mitochondrial function was determined using MTT assay.The cell morphology and the mitochondrial ultrasructure were observed by invert and transmission electron microscopy,respectively.The apoptosis of the two cell lines was determined with DNA gel electrophoresis and Annexin V and PI double-stained flow cytometry.Mitochondrial membrane potential(MMP)and the function of P-gp were assessed with rhodamine 123(Rh123)staining intensity by confocal microscopy.The release of cytochrome C from mitochondria was observed by Western blotting.Results The mitochondrial function of MCF-7/Adr was irreversibly damaged at the concentration of 4 mmol/L H2O2.The mitochondria were vacuolated and their cristae were swollen.The percentage of MCF-7/Adr cell apoptosis was significantly higher than that of MCF-7/wt,96.52% and 18.57%,respectively(P0.01).Only 800 and 1 600 Da DNA fragmentation was seen.The number of mitochondria and membrane potential of MCF-7/Adr mitochondria decreased as compared with those of MCF-7/wt.H2O2 at the concentration of 4 mmol/L induced release of cytochrome C from MCF-7/Adr mitochondria to cytoplasma,but little was detected in MCF-7/wt.Conclusion MCF-7/Adr cells expressing functional P-gp are more sensitive to H2O2-mediated mitochondrial apoptosis.It may attribute to the changes of mitochondrial structure and function in MCF-7/Adr cells.These may suggest that some way which may modify mitochondrial characteristics may be benificial to overcome the multidrug resistance related to P-gp.
【CateGory Index】: R329.28
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【References】
Chinese Journal Full-text Database 2 Hits
1 Mo Zhiqiang,Tu Gang,Luo Haojun,Li Zhenhua,Yang Guanglun(Department of Endocrine Breast Surgery,First Affiliated Hospital,Chongqing Medical University,Chongqing,400016,China);Establishment and identificantion of a Tamoxifen-resistant cell line of MCF-7 human breast cancer[J];Journal of Third Military Medical University;2011-21
2 LI Chao1,QUAN Mei-Fang2,HUANG Qiu-Lin1(1.The first affiliated hospital of nanhua university,Hengyang 421001,China;2.College of Medicine of Hunan Normal University,Changsha 410013,China);Inhibition of Viability and Induction of Apoptosis by Apigenin via Induction of Glutathione Depletion in Human Hepatocellular Carcinoma Hep G2 Cell Line[J];Journal of Hunan Normal University(Medical Sciences);2010-02
【Citations】
Chinese Journal Full-text Database 3 Hits
1 FANG Min, WANG Xiao Dong (Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, USA);The mitochondrial pathways of apoptosis[J];Journal of Beijing Medical University;2002-01
2 CAI Qian 1,ZHOU Hong 2,XIAO Guang-xia 2,et al . (1.Department of Burn and Plastic Surgery,Lanzhou General Hospital,Lanzhou Command,PLA,730050; 2. Institute of Burn Research,South-West Hospital,The Third Military Medical University,400038,China);Influence of mitochondrial membrane potential (ΔΨmt) and apoptosis in intestinal epithelia induced by hydrogen peroxide[J];Medical Journal of National Defending Forces In Northwest China;2004-04
3 CAO Guo-chun,LIANG Jun,HOU Ya-yi(1.Jiangsu Provincial Tumor Hospital,Nanjing 210009;2.Medical College of Nanjing University 210093);Coix Lacrymajobi L Varma-yuen Lipid Induces Apoptosis in MCF-7 Cell Line[J];Journal of Clinical Medicine in Practice;2007-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HE Tai-Ping1, MO Li-Er2, LIANG Nian-Ci1 1. Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang, Guangdong, 524023, P. R. China 2. Guangdong Provincial Key Laboratory for Research and Development of Natural Drugs, Zhanjiang, Guangdong, 524023, P. R. China <;Inhibitory Effect of Cantharidin on Invasion and Metastasis of Highly Metastatic Ovarian Carcinoma Cell Line HO-8910PM[J];Chinese Journal of Cancer;2005-04
2 Influence of Norcantharidin on Apoptosis of K562 Chen Jiaxu (Basic Medical School, Beijing University of Chinese Medicine, Beijing);ABSTRACTS OF ORIGINAL ARTICLES[J];China Journal of Traditional Chinese Medicine and Pharmacy;2000-02
3 ;细胞凋亡和肿瘤的关系研究进展[J];Contemporary Medicine;2009-16
4 DONG Huan-wen1,LIU Chao-mei1,LI Ke1,Lurong Zhang2,Joanne Kim21.Department of Organic Chemistry,School of Pharmacy,Second Military Medical University,Shanghai 200433,China2.Department of Radiation Oncology,URMC,601 Elmwood Ave.,University of Rochester,Rochester,NY.USA.,14642;Design,synthesis and anti-tumor activity of novel norcantharidin derivatives[J];Academic Journal of Second Military Medical University;2008-12
5 XIANG Zheng,ZHANG Cai-quan,TANG Wei-xue(Department of General Surgery,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China);Establishment and biological characteristics of human colon carcinoma multidrug-resistant cell line HT29/L-OHP[J];Acta Academiae Medicinae Militaris Tertiae;2008-09
6 Sun Zhenxiao, Zhao Tiande ** , Wei Yulin ** , Li Jiashi *** (Liaocheng Teacher's College, Liaocheng 252059; ** China Japan Friendship Institute of Clinical Medical Sciences; *** Beijing University of Traditional Chines;STUDY ON APOPTOSIS OF K562 HUMAN MYELOID LEUKEMIA CELLS INDUCED BY NORCANTHARIDIN[J];CHINESE JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY;1999-01
7 WANG Li1,XIANG Hong-lin2,CAO Jian-guo2,YANG Yong-mei1,FU Xiao-hua2,ZHENG Xing3(1.Department of anatomy,College of Medicine,University of South China,Hengyang 421001;2.College of Medicine,Hunan Normal University,Changsha 410013,China;3.Institute of Pharmacy and Pharmacology,University of South China,Hengyang 421001);creening of Genistein Derivatives for Activities of protecting Vascular Endothelial Cell injury by oxidative stress[J];Journal of Hunan Normal University(Medical Sciences);2009-03
8 HUANG Xue qiang, LING Chang quan, ZHANG Deng hai, et al (Department of TCM ,Changhai Hospital ,Second Military Medical University ,Shanghai 200433, China);Norcantharidin-induced apoptosis of cell line 6T-CEM from acute T lymphocyte leukemia[J];Basic Medical Sciences and Clinics;2002-01
9 DAI Shu-jing 1, WANG Ji-feng 2, MO Ri-gen 1, GUO Shun-gen 1, NIU Jian-zhao 1* (1. Department of Histology and Embryology, 2. Department of Biochemistry,Beijing University of Chinese Medicine,Beijing 100029,China) ;;NORCANTHARIDIN ENHANCED THG EXPRESSION OF IκBα AND INKIBITED THE PROLIFERATION OF Bel 7402 CELLS in vitro[J];Acta Anatomica Sinica;2001-02
10 LIANG Li, JIA Ting-zhen, ZHU Ying-bao, LI Ji-you. Cancer Center, Peking University Third Hospital, Beijing 100083, China;Effects of norcantharidin on the growth and apoptosis of breast cancer cell line MCF-7~(adr)[J];Chinese Clinical Oncology;2007-07
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 Leng Chao1, Liu Fei2, Yang Yuanxing1 (1The First Affiliated Hospital of Nanhua University, Hengyang, China 421001; 2College of Medicine of Hunan Normal University, Changsha, China, 410013);5, 7-dimethoxyflavone-induced apoptosis of lung cancer A540 cells is mediated by intracellular glutathione depletion[J];Anti-tumor Pharmacy;2011-02
2 Su Hua1, Liu Fei2, Yang Mingzhi1 (1The First Affiliated Hospital of Nanhua University, Hengyang, China, 421001; 2College of Medicine of Hunan Normal University, Changsha, China, 410013);Role of glutathione depletion in apigenin-induced apoptosis of osteogenic sarcoma MG-63 cell line in vitro[J];Anti-tumor Pharmacy;2011-02
3 Suai Wei1,Quan Meifang2,Huang Qiulin1*(1 The First Affiliated Hospital of Nanhua University,Hengyang,421001;2 College of Medicine of Hunan Normal University,Changsha,410013);Relationships between glutathione depletion and apigenin-induced apoptosis of pancreatic cancer PANC-1 cell line cultured in vitro[J];Anti-tumor Pharmacy;2011-03
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 HUANG Ting, WUWanyin, LI Yong, ZHANG Jiangang and HUANG Ling. 'Hangzhou Traditional Chinese Medicine Hospital, Hangzhou 310006, China;Interventional therapy combining Seed of Job's-stears with lipiodol for hepatoma-bearing rats[J];Chinese Journal of Hepatology;2002-06
2 Lu Yun, Zhang Zhongmiao, Zhang Ronghua (The First People's Hospital of Hangzhou, Hangzhou 310006);The Study on Antineoplastic Effect of Coix Seed Oil[J];PHARMACOLOGY AND CLINICS OF CHINESE MATERIA MEDICA;1999-06
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