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Establishment and evaluation of uremic atherosclerosis models in ApoE~(-/-) mice

YU Jie1,ZHAO Jing-hong2,DENG Meng-yang1,HUANG Lan1(1Institute of Cardiovascular Diseases,2Renal Diseases Research Center,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China)  
Objective To establish uremic atherosclerosis models in apoE~(-/-) mice and evaluate its feasibility.Methods Totally 70 6-week-old male apoE~(-/-) mice were randomized into 2 groups,uremic group(n=35) and nonuremic control group(n=35).All mice were fed with a Western diet.Uremic group mice underwent partial kidney ablation with electrocoagulation of right renal cortex followed by left total nephrectomy in 2 weeks later and control animals underwent sham operation.Serum levels of urea,total cholesterol and triglycerides were assessed at 0,2,4,6 and 8 weeks after the first surgery.The cross-section surface area of the lesion and the lesion area fraction were assessed.Results At 6 weeks after nephrectomy,the level of serum urea in chronic renal failure(CRF) mice was 188.3% higher than that of control mice,and total cholesterol and triglyceride concentrations were increased by 40.8% and 29.8% than those of nonuremic controls,respectively(P0.01).Aortic plaque area of CRF groups was increased 1.3-fold compared with nonuremic controls(P0.05).Moreover,the lesion area fraction of uremic apoE~(-/-) mice was markedly increased when compared with controls(P0.01).Conclusion These findings demonstrate that CRF aggravates atherosclerosis and the uremic atherosclerosis models can be established with partial kidney ablation on apoE~(-/-) mice.We anticipate that this experimental model will be useful to test treatment strategies aimed at decreasing the disease.
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