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《Acta Academiae Medicinae Militaris Tertiae》 2009-19
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Establishment and evaluation of uremic atherosclerosis models in ApoE~(-/-) mice

YU Jie1,ZHAO Jing-hong2,DENG Meng-yang1,HUANG Lan1(1Institute of Cardiovascular Diseases,2Renal Diseases Research Center,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China)  
Objective To establish uremic atherosclerosis models in apoE~(-/-) mice and evaluate its feasibility.Methods Totally 70 6-week-old male apoE~(-/-) mice were randomized into 2 groups,uremic group(n=35) and nonuremic control group(n=35).All mice were fed with a Western diet.Uremic group mice underwent partial kidney ablation with electrocoagulation of right renal cortex followed by left total nephrectomy in 2 weeks later and control animals underwent sham operation.Serum levels of urea,total cholesterol and triglycerides were assessed at 0,2,4,6 and 8 weeks after the first surgery.The cross-section surface area of the lesion and the lesion area fraction were assessed.Results At 6 weeks after nephrectomy,the level of serum urea in chronic renal failure(CRF) mice was 188.3% higher than that of control mice,and total cholesterol and triglyceride concentrations were increased by 40.8% and 29.8% than those of nonuremic controls,respectively(P0.01).Aortic plaque area of CRF groups was increased 1.3-fold compared with nonuremic controls(P0.05).Moreover,the lesion area fraction of uremic apoE~(-/-) mice was markedly increased when compared with controls(P0.01).Conclusion These findings demonstrate that CRF aggravates atherosclerosis and the uremic atherosclerosis models can be established with partial kidney ablation on apoE~(-/-) mice.We anticipate that this experimental model will be useful to test treatment strategies aimed at decreasing the disease.
【Fund】: 国家自然科学基金(30700316);; 重庆市自然科学基金(2007BB5024)~~
【CateGory Index】: R-332
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Chinese Journal Full-text Database 1 Hits
1 LIU Jian-Gang~1,DONG Guo-Ju~1,SHI Da-Zhuo~1,and WANG Yong-Yan~2 (1.Laboratory of Cardiology,Xiyuan Hospital,China Academy of TCM, Beijing 100091;2.China Academy of Traditional Chinese Medicine,Beijing 100700,China);Pathological Progress of Apolipoprotein E-Knockout Mice and the Influence of Different Diets on Atherosclerosis Progress[J];Chinese Journal of Arterial Lerosis;2005-06
Chinese Journal Full-text Database 8 Hits
1 ZHAO Hai-feng1,2,ZHANG Zhao-feng1,LI Qiong1,ZHAO Ming1,LI Yong1(1.Department of Nutrition and Food Hygiene,School of Public Health,Beijing University,Beijing 100083;2.Department of Nutrition and Food Hygiene,School of Public Health,Shanxi Medical University,Taiyuan 030001,China);Adenine-induced Chronic Renal Function Impairment in Rats[J];Carcinogenesis,Teratogenesis & Mutagenesis;2008-05
2 GENG Jing(Department of Chinese Medicine,Renmin Hospital of Huaibei City,AnHui Province,Anhui Huaibei 235000,China);Clinical Observation on Chinese Medicinal Herbal Retention-enema to Treat 66 Cases of Chronic Renal Failure[J];Journal of Anhui Traditional Chinese Medical College;2006-01
3 MA Wen-bo1,GAO Xin-chun2,WANG Jian-yi1,YANG Shu-wen3 (1.Department of Urology Surgery,The Frist Hospital of Zhangjiakou,Zhangjiakou Hebei 075000;2.First Affiliated Hospital,Hebei North University,Zhangjiakou Hebei 075000;3.Second Affiliated Hospital,Hebei Medical University,Shijiazhuang Hebei 050061,China);Effect of Tiaoqi Huoxue Bushen decoction on experimental renal injury in rats[J];Journal of Bengbu Medical College;2008-04
4 ZHANG Yao-quan,FENG Bing,YUAN Fa-huan(Renal Diseases Research Center of PLA,Department of Nephrology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China);Establishment of the rabbit model of chronic renal failure by the branchs′ deligation of arteria renalis[J];Chongqing Medicine;2008-18
5 LI Dan,ZHAO Xue-zhi~*(Department of Nephrology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China);High phosphorus foods combined with 5/6 nephrectomy for establishing rat hyperparathyroidism model secondary to chronic renal failure[J];Academic Journal of Second Military Medical University;2006-07
6 XIE Jin-jie,YANG Ya(Dept. of Ultrasound, Anzhen Hospital of Capital Medical University, Beijing 100029, China);Application of gene-knocking out mice in the research of atherosclerosis[J];Basic & Clinical Medicine;2008-04
7 ZHANG Yaoquan,FENG Bing,Li Furong,et al Renal Diseases Research Center of PLA,Department of Nephrology,Xinqiao Hospital,Third Military Medical University,Chongqing(400037);Observation of Accelerated Atherosclerotic Pathomorphology of the Rabbit Model of Chronic Renal Failure[J];Chinese Journal of Integrated Traditional and Western Nephrology;2008-06
8 ZHOU Jing, AI Min, WANG Zhao-yang (Department of Pharmacology, Inner Mongolia Medical College, Hohhot 010059 China);DISEASE MODEL OF CHRONIC RENAL FAILURE IN RATS MADE BY ADENINE[J];Acta Academiae Medicinae Neimongol;2004-04
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Yuan Fahuan , Liao Lisheng (Department of Nephrology, Xinqiao Hospital, Third Military Medical College, Chongqing, 630037);Establishment and evaluation of the cadmium-induced mouse model of chronic renal failure[J];Acta Academiae Medicinae Militaris Tertiae;1993-06
2 Wang Yaoguang The Second Affiliated Hospital of Tianjing College of TCM(300150);A Primary Approach to the Nature of Blood Stasis of Animal Model with Chronic Renal Failure Caused by Electric Cauterization[J];HENAN TRADITIONAL CHINESE MEDICINE;1997-01
3 Zhou Xiaozhou ;Zhang Shengguang; Yang Xiao; Wei Yi (Hunan College of TCM, Changsha, China410007);Study on Mechanism of Adenine-Induced Chronic Renal Failure in Rat[J];BASIC MEDICAL SCIENCES AND CLINICS;1997-01
4 Lin Na, Chen Ronghua, Huang Songming, et al. Center of Pediatric Nephrology, Nanjing Medical University, Nanjing 210029;Modified Animal Model of Rapid Glomerulosclerosis[J];ACTA ACADEMIAE MEDICINAE NANJING;2000-03
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