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《Acta Academiae Medicinae Militaris Tertiae》 2010-01
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Detection and clinical significance of CEA,GCC and CD44v6 mRNA in peritoneal lavages of colorectal cancer patients:report of 60 cases

Wang Haobin1,Zhang Caiquan1,Wang Chongshu2,Zhao Mingcai3,Xie Xianyong4,Hou Huafang2(1Department of Gene-ral Surgery,First Affiliated Hospital,Chongqing Medical University,Chongqing,400016;2First Department of General Surgery,4Department of Pathology,Affiliated Hospital of North Sichuan Medical College,3Research Institute of Rheumatology and Immunology,North Sichuan Medical College,Nanchong,Sichuan Province,637000,China)  
Objective To explore the significance of the transcripts of carcinoembryonic antigen(CEA),guanylyl cyclase C(GCC) and metastasis-related gene CD44v6 in peritoneal lavages of colorectal cancer patients.Methods The peritoneal lavages of 48 patients with identified colorectal cancer(colorectal cancer group) and 12 patients with benign colorectal disease(negative control group) were collected.All of the patients were pathologically confirmed.Human colorectal cancer cell line SW480 were taken as a positive control.The mRNA levels of CEA,GCC and CD44v6 in the collected cells were detected by QuantiGene Reagent System,which is a quantitative RNA detection method based on branched DNA(b-DNA) signal amplification technique.Meanwhile,cytologic examination with microscopy was performed.Results The positive expression rates of CEA,GCC and CD44v6 in the peritoneal lavages were 43.8%(21/48),60.4%(29/48) and 31.3%(15/48) respectively,and the total positive rate was 72.9%(35/48) in colorectal cancer group,which was more sensitive than that of cytologic examination(2%,1/48).All 3 marker genes were expressed in human colorectal cancer cell line SW480.There was only 1 sample positive to CEA mRNA in the negative control group.The positive rate of CEA,GCC and CD44v6 expressions were correlated with the Dukes staging,differentiation status,serosal involvement and lymph node metastasis(P0.05),but not with tumor size,patient age and gender(P0.05).Conclusion The detection of CEA,GCC and CD44v6 mRNA in peritoneal lavages is helpful for early diagnosis,prognosis assessment and suitable treatment selection for colorectal carcinoma.Similar to CEA,GCC and CD44v6 may be used as indicators for peritoneal micrometastasis of colorectal cancer.The combined application of these 3 molecular markers will improve the positive detection rate of peritoneal micrometastasis.The b-DNA signal amplification technique-based mRNA detection is more sensitive and specific than that of cytologic examination,and is an effective way to predict peritoneal micrometastasis.
【Fund】: 四川省科技厅应用基础研究项目(04JY029-015-3)~~
【CateGory Index】: R735.34
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