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《Acta Academiae Medicinae Militaris Tertiae》 2010-18
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Apoptosis and necrosis of HeLa cells induced by irreversible electroporation

Zhou Wei1,Xiong Zheng'ai1,Liu Ying1,Li Chengxiang2,Yao Chenguo2(1Department of Gynecology and Obstetrics,Second Affiliated Hospital,Chongqing Medical University,Chongqing,400010;2State Key Laboratory of Power Transmission Equipment and System Security and New Technology,Chongqing University,Chongqing,400030,China)  
Objective To investigate the impact of pulse electric fields(PEFs)on membrane breakdown,necrosis and apoptosis of HeLa cells when irreversible electroporation(IRE)was performed on it.Methods PEFs with a permanent duration of 100 μs,a frequency of 1 Hz and pulse of 8 pieces were performed on HeLa cells(2×106/ml).The electric field intensity was increased from 250 V/cm to 2 500 V/cm gradually with 250 V/cm for each time.Propidium Iodide(PI)was used to trace the breakdown of cell membrane with the aid of flow cytometry according to the manufacturer's protocol.Cell counting kit-8(CCK-8)assay was used to examine survival of HeLa cells after treated with IRE.HeLa cells were double-stained with Annexin V kit then analyzed with flow cytometry for apoptosis and necrosis.Results The reversible electroporation threshold of HeLa cells was 750 V/cm.The apoptotic threshold ranged from 1 250 V/cm to 1 500 V/cm.The necrotic threshold was about 1 750V/cm.Conclusion Besides necrosis due to IRE,apoptosis also played an important role during IRE ablating HeLa cells.The areas of PEF parameters inducing apoptosis and necrosis are overlapped with each other.
【Fund】: 国家自然科学基金重点项目(50637020);; 重庆市自然科学基金重点项目(CSTC2009BA5039)~~
【CateGory Index】: R737.33
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