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Effect of static magnetic field at different strengths on Ca~(2+) concentration in rat primarily cultured osteoblasts

Wang Shengguo1, Zhou Li2 , Chen Yangxi2, Wang Yanmin2, Ren Xiaohua3 (1Department of Stomatology, Second Affiliated Hospital , Chongqing Medical University, Chongqing, 400010; 2Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province, 610041; 3 Department of Stomatology , People’s Hospital of Sichuan Province, Chengdu, Sichuan Province, 610072, China)  
Objective To investigate the Ca2+ concentration in osteoblasts and to study its changing rule during static magnetic field loading. Methods Osteoblasts primarily cultured form 1-day rat skull were cultured under 0, 8, 50 and 160 mT of static magnetic field for 24, 48 and 72 h, respectively. The cells cultured under 0 mT used as control. Osteoblasts were identified with alkaline phosphatase (ALP) and Von Kossa stainings. Ca2+ concentrations were measured with the OCPC micro-plating method and analyzed by variance. Results The culuted cells showed the biological features of osteoblasts and positive to ALP and Von Kossa stainings. Compared with the cells under 0 mT field, the Ca2+concentration of 8 mT group was decreased by 27.48%, 24.41% and 10.46% respectively at 24, 48 and 72 h, that of 50 mT group was by 19.03%, 18.04% and 15.54%, and that of 160 mT group was by 8.58%, 13.10% and 19.03% respectively for different time points. Significant reduce was found in the Ca2+concentration after static magnetic field exposure compared with that of normal control (P0.05). Conclusion The static magnetic field at a certain strength can decrease the Ca2+concentration in osteoblasts.
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