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《Journal of Third Military Medical University》 2011-22
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Effect of cholecystokinin octapeptide on IL-1β,IL-6 and IL-10 expression in LPS-induced RAW 264.7 cells

Ni Zhiyu,Cong Bin,Dong Mei,Ma Chunling,Li Shujin,Wen Di,Xu Zhilu(Department of Forensic Medicine,Hebei Key Laboratory of Forensic Medicine,College of Basic Medicine,Hebei Medical University,Shijiazhuang,Hebei Province,050017,China)  
Objective To observe the dynamic changes of proinflammatory cytokines IL-1β,IL-6 and anti-inflammatory cytokines IL-10 in LPS-induced RAW 264.7 cells and observe the effects of cholecystokinin octapeptide(CCK-8) on their expression.Methods After RAW 264.7 cells were stimulated with 1 mg/L LPS for 0,3,6,12,24 and 48 h respectively,the expression peak times of IL-1β,IL-6 and IL-10 were selected to investigate the effects of CCK-8 at the doses of 10-10,10-9,10-8,10-7 and 10-6 mol/L respectively.Cytokines protein and mRNA expressions were assayed by ELISA and RT-PCR.Results The expression of IL-1β,IL-6 and IL-10 mRNA and protein were induced by LPS in RAW 264.7 cells in a time-dependent manner.CCK-8 inhibited LPS-induced IL-1β and IL-6 expression and augmented IL-10 expression in a dose-dependent manner.When the cells were treated for 24 h,CCK-8 augmentation resulted in higher LPS-induced IL-10 expression when compared with that after 48 hours' treatment.Conclusion CCK-8 may participate in the anti-inflammatory response by inhibiting the expression of proinflammatory cytokines IL-1β and IL-6,meanwhile augmenting the expression of anti-inflammatory cytokine IL-10 induced by LPS.
【Fund】: 国家自然科学基金面上项目(30770839);; 河北省自然科学基金(C2008001045)~~
【CateGory Index】: R965
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