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《Journal of Third Military Medical University》 2012-23
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Role of transient receptor potential channels 6 in iron metabolism in rats after traumatic brain injury

Zhang Lijun,Hu Rong,Li Fei,Meng Hui,Lin Jiangkai,Zhu Gang,Wang Xianrong,Feng Hua(Department of Neurosurgery,Institute of Neurosurgery,Key Laboratory of Neurotrauma Prevention and Treatment,Southwest Hospital,Third Military Medical University,Chongqing,400038,China)  
Objective To determine the role of transient receptor potential channels 6(TRPC6) in iron metabolism after traumatic brain injury(TBI) in rats and investigate the underlying mechanism.Methods Male Sprague-Dawley rats were randomly divided into 3 groups(n=13 for each group),that is,sham-operation group,TBI group and deferoxamine(DFO) treatment group.Modified Feeney's method was used to establish the rat model of experimental TBI with an impact of a weight of 30 g from 15 cm after craniotomy.The rats of DFO treatment group were treated with DFO(100 mg/kg,i.p.,administered 2 h after TBI and then at 12-hour intervals for up to 28 d).Nonhemoglobin iron around the injury site was measured.Immunofluorescence staining was used to detect the expression and location of TRPC6 and NeuN,and Western blotting was also used to detect the expression of TRPC6.Results The volumes of nonhemoglobin brain iron was significantly decreased in injured group and DFO treatment group than sham-operation group(P0.05),but there was no difference between TBI group and DFO treatment group.The expression of TRPC6 was up-regulated significantly after TBI compared with sham-operation group.DFO administration significantly down-regulated the expression of TRPC6.After TBI,there were more neurons positive to TRPC6,but few positive to NeuN around the injury site.However,DFO treatment resulted in more neurons positive to NeuN,which was coexpressed with TRPC6.Conclusion Over-loading of iron exerts neurotoxic effect in rats after TBI,which may deteriorate the damage of neurons around injury site.TRPC6 maybe play an important role in the neurotoxicity of the process.
【Fund】: 国家自然科学基金(30872674 30901545)~~
【CateGory Index】: R651.1
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 GONG Guo-qi,MENG Hui,XIA Yong-zhi,FENG Hua(Department of Neurosurgery,Southwest Hospital,Third Military Medicine University,Chongqing 400038,China);Experimental chronic hydrocephalus after intraventricular hemorrhage in rats:establishment of animal model and pathological observation[J];Acta Academiae Medicinae Militaris Tertiae;2008-10
2 GONG Guo-qi,MENG Hui,FENG Hua (Department of Neurosurgery,Southwest Hospital,Third Military Medicine University,Chongqing 400038,China);Role of iron ion in development of hydrocephalus in rats[J];Acta Academiae Medicinae Militaris Tertiae;2008-23
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 CHEN Jing-yu,MENG Hui,FENG Hua(Department of Neurosurgery,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);TRP channel and central nervous system diseases[J];Journal of Traumatic Surgery;2010-02
2 GONG Guo-qi,MENG Hui,FENG Hua (Department of Neurosurgery,Southwest Hospital,Third Military Medicine University,Chongqing 400038,China);Role of iron ion in development of hydrocephalus in rats[J];Acta Academiae Medicinae Militaris Tertiae;2008-23
3 Zhang Lijun,Hu Rong,Li Fei,Meng Hui,Lin Jiangkai,Zhu Gang,Wang Xianrong,Feng Hua(Department of Neurosurgery,Institute of Neurosurgery,Key Laboratory of Neurotrauma Prevention and Treatment,Southwest Hospital,Third Military Medical University,Chongqing,400038,China);Deferoxamine attenuates traumatic brain injury in rats[J];Journal of Third Military Medical University;2012-23
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