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《Journal of Third Military Medical University》 2013-06
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Curcumin suppresses proliferation and induces apoptosis in human medulloblastoma cells via PI3k/Akt signaling pathway

Mao Shu1,3,He Misi1,3,Liu Guiyuan1,3,Zhang Xiong2,3,Li Yu2,3,Tang Li1,3(1Department of Pathophysiology,2Department of Pathology,3Institute of Neuroscience,College of Basic Medical Sciences,Chongqing Medical University,Chongqing,400016,China)  
Objective To determine the role of curcumin to PI3k/Akt signaling pathway in human medulloblastoma.Methods Daoy cells were treated with curcumin at different concentrations of 20 to 100 μmol/L for time intervals of 24,48,and 72 h when they were in the logarithmic growth phase.The proliferation and apoptosis in Daoy cells in presence or absence of curcumin was analyzed by MTT assay and flow cytometry,respectively.The expression of PI3K,Akt and p-Akt at mRNA and protein levels was detected by RT-PCR,immunocytochemistry and Western blotting,respectively.Results Exposure of the cells to curcumin resulted in a decrease in cell proliferation in a dose-and time-dependent manner(P0.05).When the concentration of curcumin was 35 μmol/L and the treatment was for 48 h,the inhibitory rates reached peak and a significant increase of cell apoptosis was observed.The positive expression of PI3K,Akt and p-Akt in the cells without treatment were 80.7%,84.8% and 87.5%,respectively,which were significantly higher than those in the curcumin group(25.3%,58.8% and 26.7%,respectively,P0.05).The relative expression of PI3K,Akt and p-Akt protein in the cells without treatment were 1.141±0.032,1.047±0.174 and 1.173±0.013,respectively,with those of the first and last proteins were significantly higher than those of the curcumin group(0.875±0.029 and 0.958±0.150,P0.05).The relative expression of PI3K and Akt mRNA in the cells without treatment were 1.166±0.031 and 1.072±0.007,respectively,with the expression of PI3K were significantly higher than that of the curcumin group(0.833±0.033,t=12.468,P0.05).Conclusion Curcumin inhibits cell proliferation and induces apoptosis in medulloblastoma Daoy cells through the PI3K/Akt signaling pathway.
【Fund】: 国家自然科学基金面上项目(81272571);; 重庆市自然科学基金(CSTC2009BB5064);; 重庆市渝中区科技计划项目(20120208)~~
【CateGory Index】: R739.4;R285.5
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【Citations】
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1 LIN Xiao,LIN Qing,TANG Li,LIU Bin,LI Yu(Institute of Neuroscience,Chongqing Medical University,Chongqing 400016,China);Expressions of PI3K,AKT and Nrf2 in Medulloblastoma and Relevant Significances[J];Chinese Journal of Biologicals;2011-09
2 Wang Jingyu,Wang Zhiping 1,Zhao Junli 1,Zhang Zhewen (Institute of Pathophysiology,School of Basic Medical Sciences,Lanzhou University,Lanzhou 730000; 1 Institute of Urology,Second Hospital,Lanzhou University,Lanzhou 730030);Curcumin induces Apoptosis via PTEN/PI3K/Akt pathway in human bladder careinoma EJ cells[J];Pharmacology and Clinics of Chinese Materia Medica;2011-01
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1 Jinqiang ZHANG*,Yuhong MENG*#,Huiyun LIN*,Gang LIU*,Yan WANG*,Huihua CHEN*,Yuanji XU*,Zhiyan DU*,Yinglin LU* *Department of Pathobiology,Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850,China; #Present address:Department of Pathology,Navy General Hospital,Beijing 100037,China;Promotion of MAG-1 on Metastasis of Lung Cancer Cells in vitro and Its Expression in Lung Cancer Tissue of 24 Cases[J];Chinese Journal of Lung Cancer;2009-02
2 LIU peng-qi1,DING Shu-zhe2,SHAO Yue3(1.Dept.of P.E.,Henan Judicial Police Vocational College,Zhengzhou 450011,China;2.College of P.E.,East China Normal University,shanghai 200062,China;3.Dept.of P.E.,Henan Politics and Law College of Management Cadre,Zhengzhou 450002,China);The Possible Mechanism of the P53 Regulate the Energy on Mitochondrial and Adaptating to the Movement[J];Journal of Guangzhou Sport University;2008-03
3 ZHANG Xiu-juan1,2,HAN Lei1,2,LING Li-li1,2,LI Qiu-shi1,2,JI Yu-bin1,2,FANG Gui-zhen3(1.Postdoctoral Research Station of Institute of Medicine of Center of Research and Development on Life and Environment Science,Harbin University of Commerce,Harbin 150076,China;2.Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin 150076,China;3.College of Material and Engineering,Noethest Forestry University,Harbin 150040,China);Study of betulonic acid on anti-tumor by inhibiting PI3K/AKT pathways[J];Journal of Harbin University of Commerce(Natural Sciences Edition);2008-03
4 MU Ling-mina,WANG Wen-fengb,GUO Zhi-kunc,ZHANG Guang-moub(a.Morphologial Laboratory;b.Life Science and Technique Department;c.The Key Laboratory for Tissue Regeneration of Henan Universities,Xinxiang Medical College,Xinxiang 453003,China);Influence of PI3K and PTEN's Expression in Rats' Myocardial Hypertrophy for Myocardial Fibrosis[J];Journal of Henan Normal University(Natural Science Edition);2012-03
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1 GAO Wei,ZHANG De-ping,HUA Yun-feng. Department of Respiratory Medcine, Affiliated Nanjing Drum Tower Hospital of Nanjing University, Nanjing 210000, China;Effect of Curcumin on proliferation and apoptosis of pulmonary fibroblasts[J];The Journal of Practical Medicine;2008-14
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