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《Journal of Third Military Medical University》 2013-12
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Protective effect of icariin on radiation damage of Laydig cells in rats

Zhang Weixing,Jia Donghui,Wang Rui,Li Zhou,Chi Kaikai(Department of Urology,First Affiliated Hospital of Zhengzhou University,Open Laboratory of Key Disciplines of Henan Clinical Medicine,Zhengzhou,Henan Province,450052,China)  
Objective To study the protective effect of icariin(ICA) on 60Co-γ-induced damage of Laydig cells in rats.Methods Fifty male Wistar rats were randomly divided into five groups with 10 in each group including Group A(control),Group B(radiation control),Group C(ICA irrigation for 3 d),Group D(ICA irrigation for 7 d),and Group E(ICA irrigation for 15 d).The rats of Group A and Group B were irrigated with normal saline for 15 d,while those of Group C,Group D and Group E were irrigated with ICA [80 mg/(kg·d)] for 3,7 and 15 d before irradiation,respectively.Then the lower abdomen of rats in Group B,Group C,Group D and Group E were exposed to the uniform radiation of 60Co-γ with 8 Gy.All the animals were sacrificed 12 h later.The histological observation of the testis tissues were performed by microscopy,the levels of testosterone(T) and luteinizing hormone(LH) were determined by ELISA,and the expression of P450scc mRNA was determined by RT-PCR.Results Compared with Group A and Group B had a decreasing tendency for T(P0.05) and an increasing tendency for LH(P0.05).Group C,Group D and Group E all had increasing trends for T and decreasing trends for LH as compared with Group B,and the difference between Group B and Group E was statistically significant(P0.05).The expression of P450scc mRNA in Group B was significantly lower than that in Group A(P0.05).Compared with group B,the expression of P450scc mRNA increased in Group C,Group D and Group E,and the difference between Group B and Group E was statistically significant(P0.05).Conclusion Traditional Chinese medicine monomer of ICA has a protective effect on radiation damage of Laydig cells in rats,especially in the group of ICA irrigation for 15 d.
【Key Words】: radiation icariin Laydig cell
【Fund】: 河南省科技攻关重点项目(102102310067)~~
【CateGory Index】: R285.5
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