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《ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE》 1999-01
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Optimizing of RAPD PCR procedure in mice

Wu Fengchun, Wei Hong (Laboratory Animal Center, Third Military Medical University, Chongqing, 400038)  
Objective: To optimize the RAPD PCR procedure in mice. Methods: Forty primers were used to amplify DNA in 9 strains of mice and then the results of the amplification were analyzed. Results: Optimal reaction system was as follows: 25 μl of each PCR mixture containing 2.5 μl of 10× Taq Reaction buffer, 200 μmol/L of each dNTPs, 2.5 mmo/L of Mg 2+ , 0.2 μmol/L of primer, 1.5 unit of Taq DNA polymerase and 20 ng of total DNA. The reaction condition was 40 cycles of denaturation at 94℃ for 1 min, annealing at 38℃ for 1 min and extension at 72℃ for 2 min. Conclusion: The use of the above mentioned reaction system and amplifying procedure for RAPD PCR in mice can get stable results.
【Fund】: 国家自然科学基金 全军“九五”医药卫生科研基金 重庆市攻关项目
【CateGory Index】: Q550.3
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