Preparation and immunological characterization of glioma basic protein-specific immune RNA
HUI Hong-Xiang and WANG Cheng-Ji Department of Biochemistry
Immune RNA (iRNA) was prepared from the lymphoid tissue of guinea pigs immunized with glioma basic protein (GBP) extracted from pooled human glioma tissues, and its immunoactivity was verified by 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyl tetrazolium bromide-leucocyte adherence inhibition (MTT-LAI), a non-radioisotopic version of leucocyte adherence inhibition assay. The results showed that GBP-specific iRNA could mediate LAI in response to brain tumor antigens, such as glioma and meningioma antigens. 3H-Leu-LAI, currently used in cell-mediated immunity assay in vitm, could be replaced by MTT-LAI.