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《Journal of The Fourth Military Medical University》 1993-06
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Two-step multiplex polymerase chain reaction for gene diagnosis of progressive pseudohypertrophic muscular dystrophy

TAN Qing-Rong,WU Bao-Ren and WANG Lian-Gang Department of Neurology,Xijing Hospital  
In the present study. 9 oligonucleotide primers were employed to amplify 9 pairs of DNA sequences of dystrophia gene using two-step multiplex polymerase chain reaction (mPCR). Furthermore, gene diagnosis was undertaken of 19 cases of Duchenne muscular dystrophy (DMD) and 2 cases of Becker muscular dystrophy. The findings showed that 8 cases of deletion were detected by 5 amplified primers with a relatively high incidence of deletion and 2 more cases of deletion were detected using the remaining 4 amplified primers. The deletion rate of 9 primers using two-step mPCR was 47. 2% of the patients examined, suggesting that about 90% of patients with gene deletion were delected. The results of the experiment show that two step mPCR can be used in the gene diagnosis of DMD/BMD. This method is not only simple, convenient and rapid, but also free radioisotope interforence. Therefore, this method may be of importance for rapid diagnosis of DMD/BMD.
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Yin Xuejun, Wang Hechuan. Liu Dexiang Laboratory of Medical Genetics, Wulumaqi General Hospital Lanzhou Command, Xinjiang 83000;INTEGRATE STUDY ON THE CRITERIA OF INDICATION OF THE GENETIC SAFETY IN 271 NORMAL INDIVIDUALS[J];Carcinogenesis,Teratogenesis and Mutagenesis;1992-05
2 Xu Jinlin;A Kindred of Peroneal Muscular Atrophy[J];Journal of Anhui University(Natural Sciences);1989-03
3 Lu'an People's Hospital, Lu'an 237005, Anhui Chen Wei-qing, Chen De-gang, Xiang Ju-le;Report on a family tree of adult polycystic[J];Journal of Anhui Health Vocational & Technical College;2006-01
4 Lu Xiaowei; Lin Zhenyu(1 The Vanguard Physical Culture and Sports Association of china,100741)(2 Research Institute,BUPE,Beijing 100084);Dactylograph Characteristics of Chinese Top Basketballers[J];Journal of Beijing University of Physical Education;1995-01
5 ZHOU Xianmei;MA Shixiong;LIU Chunlan;YU Chang;TANG Suen;ZHAO Rui;et al(Department of Obstetrics and Gynecology,Third Hospital, Beijing Medical University 100083);NUCLEAR DNA CONTENT AND IMAGE ANALYSIS OF CRVICALINTRAEPITHELIAL NEOPLASIA CERVICAL,CANCER AND CONDYLOMA ACUMINATE[J];JOURNAL OF BEIJING MEDICAL UNIVERSITY;1996-05
6 Ge Ruling Department of Biology;A Preliminary Report to Changing Law of Dermatoglyph in Schizophrenic Fatients[J];Journal of Binzhou Medical College;1987-02
7 Ge Ruling;ANALYSIS OF A PEDIGREE OF POLYDACTYLY AND SYNDACTYLY Department of Biology Binzhon Medical College.[J];Journal of Binzhou Medical College;1987-04
8 Ge Ruling, et al Department of Biology, Binzhou Medical College, Shandong Province;STUDIES OF CHANGING LAW OF DERMATOGLYPH IN SCHIZOPHRENIC PATIENTS[J];Journal of Binzhou Medical College;1989-04
9 Mei Qixia,et al. (Dept. of Clinical Psychology,Children's Hospital of Chongqing Medical University, Chongqing 400014);The Analysis of Chromosomes of 207 Children with Congenital Mental Retardation.[J];CHONGQING MEDICAL JOURNAL;2000-02
10 Xu Yanchun(Northeast Forestry University. Harbin 150040. P. R. China); Ma Lixin(Heitu Trade Limited-liability Company of Heilongjiang); Bai Suying; Jin Yu; Jing Songyan(Northeast Forestry University);Application of PCR to Mammalian Gender identification with Hair[J];Journal of Northeast Forestry University;2000-06
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