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Aspiration of rabbit bone marrow by bone puncture

LIU Dan Ping 1, HU Yun Yu 1, SHI Xin You 2, SUN Long 1, MA Sheng Bing 1 1Institute of Orthopedics of Chinese PLA, Xijing Hospital, 2Laboratory Aminal Research Center, Fourth Military Medical University, Xi'an 710033, China  
AIM To probe into better methods to obtain bone marrow (BM) by bone puncture and aspiration from rabbit bones. METHODS: Locations and extents of puncture points(PPs) at rabbit hind limb bones suitable for aspiration of BM were determined by preparation of the skeleton specimen, observations of the topographic features and the size and distribution of BM cavities of these bones and utilization of landmarks on their body surface. RESULTS Four puncturepoints (PPs) suitable for aspiration of BM on the hip bone, femur and tibia were proposed. They were:①ischium PP: at the middle point between tuberosity of ischium and lateral process of ischial tuberosity; ②superior femoral PP: In the extent of 0.3~0.5 cm below the third trochanter apex of femur; ③inferior femoral PP: In the extent of 1.0~1.5 cm of lateral bone surface up the space of knee joint; ④anterointernal tibial PP: In the extent of 1.0~2.5 cm of anterointernal bone surface below the space of knee joint at the upper part of tibia. In the present paper the author's experience of bone puncture and aspiration of BM at the four PPs was also introduced. CONCLUSION Manipulated according to the procedures and regulations proposed in the present paper, rabbit BM can easily be aspirated from the four points.
【CateGory Index】: Q95-3
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