Effects of psoralen and 8 methoxypsoralen on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells

WU Jun Zheng, SI TU Zhen Qiang, CHEN Jian Yuan, LI Feng, LI Yan, LIU Bin Department of Oral Biology, Qindu Stomatological College, Fourth Military Medical University, Xi'an 710033, China [WT5”,6HZ]  

AIM To study the effects of psoralen (PSO) and 8 methoxypsoralen (8 MOP) on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells. METHODS Inhibitory effects of PSO and 8 MOP on the proliferation and metastatic potential of mucoepidermaid carcinoma Mc3 cells were investigated with MTT assay, cell counting, flow cytometry and tail vein injection of the cells into nude mice (10 6 for each). RESULTS PSO and 8 MOP inhibited Mc3 cell growth in a dose dependent way. The IC 30 (mg·L -1 ) of PSO and 8 MOP were 32.4 and 25.1 and IC 50 (mg·L -1 ) 44.7 and 35.5 respectively. After the cells had been treated with the drugs at IC 30 for 5 d, the doubling time (h) for the control, PSO treated and 8 MOP treated cells were 20.8, 23.3 and 57.8 respectively, the percentage of S phase cells 24.5,17.8 and 5.8, the wild type p53 expression (%) 24.0,99.8 and 99.0, the nm23 H 1 expression (%) 99 9,99.5 and 99.1, the clonogenesity (%) 23.5,16.5 and 0, the number of metastatic foci on lung surface 139±61,114±68 and 36±32, respectively. CONCLUSION Both PSO and 8 MOP at the dosage of IC 30 may inhibit the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells, but 8 MOP is more effective.

【Key Words】： salivary gland mucoepidermoid carcinoma neoplasm metastasis psoralen 8 methoxypsoralen
【Fund】： 第四军医大学科技创新工程资助!项目 ( CX99A0 12 )
【CateGory Index】： R73-36

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