Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of The Fourth Military Medical University》 2001-06
Add to Favorite Get Latest Update

Protein quantification with coomassie bulliant blue microplate-colormetric

WANG Duo Ning 1, ZHAO Yan Wu2, TIAN FuRong3 1Department of Basic Medicine, Shaanxi Medical College, Xi'an 710068, China, 2Department of Chemistry, Faculty of Preclinical Medicine, 3Department of Radiomedicine, Faculty of Preventive Medicine,  
AIM To develop a rapid and sensitive method of protein quantification assay. METHODS Protein content of bovine serum albumin (BSA) and mice liver homogenate was determinated by microplate colormetric (MPC) in which coomassie brilliant blue (CBB) is used as color reagent. RESULTS Detecting limit of MPC was lower (0.63 μg) than that of macro colormetric (MC). MPC had advantage of rapidity and lesser volume of sample (a few microliter). CONCLUSION MPC is a method of simplicity, rapidity and sensitivity for protein quantification assay especially suitable for the determination of large number of microliter samples.
【CateGory Index】: Q503
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 LUAN Ji-mei, HU Ming-jin, ZHANG Xiao-dong (Chemical College of Qingdao University, Qingdao 266071, China);The Study on Protein Determination through the Spectrophotometric Analysis by Means of Dyes[J];Journal of Anhui Institute of Education;2004-03
2 HE Tai-Ping1, MO Li-Er2, LIANG Nian-Ci1 1. Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang, Guangdong, 524023, P. R. China 2. Guangdong Provincial Key Laboratory for Research and Development of Natural Drugs, Zhanjiang, Guangdong, 524023, P. R. China <;Inhibitory Effect of Cantharidin on Invasion and Metastasis of Highly Metastatic Ovarian Carcinoma Cell Line HO-8910PM[J];Chinese Journal of Cancer;2005-04
3 ;Investigation Of Protein Adsorption By Quartz Crystal Microbalance[J];Sensor World;2007-12
4 LIU Yong-Ying1,2, WANG Duo-Ning3, LIU Yuan-Sheng1,YANG Ying3,ZHAO Yan-Wu4,WANG Zeng-Lu5, YANG Ji-Qing11Department of Physics, School of Biomedical Engineering, Fourth Military Medical University, Xi'an 710033, China, 2Xi'an Yanliang 630 Hospital, Xi'an 710089, China, 3Scientific Research Center, Xi'an Medical College, Xi'an 710068, China, 4Economical Department, Engineering College of Armed Police Force, Xi'an 710086, China, 5Center for Biotechnology, Fourth Military Medical University, Xi'an 710033, China;Preventive and therapeutical effect of the kernel of zanthoxylum seed oil on experimental hyperlipidemia in rats[J];Journal of the Fourth Military Medical University;2007-05
5 WANG Duo-Ning1,YANG Ying1,ZHAO Yan-Wu2,MI Man1,MEI Qi-Bing3,SHI Ming-Juan1,SU Xing-Li1,SHANG Ya-Juan1,LIU Yong-Ying4 1Scientific Research Center,Xi'an Medical University,Xi'an 710021,China,2Department of Economics,Engineering College of Chinese People's Armed Police Forces,Xi'an 710086,China, 3Department of Pharmacy,Fourth Military Medical University,Xi'an 710033,China,4Xi'an Yanliang 630 Hospital,Xi'an 710089,China;Double-scavenging activities of protocatechualdehyde-thiosemicarbazone-copper(Ⅱ)complex to superoxide free radical and hydroxyl free radical[J];Journal of the Fourth Military Medical University;2007-14
6 ZHAO Yan-Wu1,2,QIU Nong-Xue1,GAO Xia1,WANG Duoning3,YANG Ying3 1 College of Life Sciences,Shaanxi Normal University,Xi'an 710062,2Economical Department,Engineering College of People's Armed Police Forces,Xi'an 710086,3Scientific Research Center,Xi'an Medical University,Xi'an 710068,China;Preventive and therapeutical effect of Apple seed oil on experimental hyperlipidemia in rats[J];Journal of the Fourth Military Medical University;2008-06
7 LI Zhi-min,WANG Bo-chu,ZHOU Jing, PENG Liang nistry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China);Comparison of three methods of removing protein from polysaccharide extract in the plant[J];Journal of Chongqing University(Natural Science Edition);2004-08
8 ZHOU Jing,WANG Bo-chu,WANG Yang,LI Zhi-min(Key Laboratory for Biomechanics & Tissue Engineering Under the State Ministry of Education,College of Bioengineering,Chongqing University,Chongqing 400030,China);Technologic Study on the Isolation of Polysaccharides from Herba Spirodelae seu Lemnae by Ultrafiltration[J];Journal of Chongqing University(Natural Science Edition);2006-04
9 HE Tai-ping, HE Zhen-hui, MO Li-er, LIANG Nian-ci (Department of Biochemistry, Guangdong Medical College , Zhanjiang 524023, China);Effects of cantharidin on the expression of NF-κB(P65) and Smad3 genes in cells of ovarian carcinoma cell line HO-8910PM with highly metastatic potential[J];Journal of Guangdong Medical College;2005-02
10 Shi De-fang1,Yang Yang2,Sun Xiao-xue1,Ren Hong1,Han Shu-qin1 (1.College of Light Industry and Food Engineering,2.College of Life Science,Guangxi University,Nanning 530004);Comparison of three Methods of Removing Protein from Polysaccharide Extract in the Cactus[J];Modern Food Science and Technology;2006-04
China Proceedings of conference Full-text Database 2 Hits
1 HE Zhi-wei1 LIU Zhong-dong1 SHAO Bin2 WEI Guo-hua1 XU Xin-de2(1.School of Oil and Food Henan University of Technology, Zhengzhou 450052,China;2.Zhejiang Xinchang Pharmaceutical Factory,Xinchang 312500,China);Study on the removed protein of vaccinium bractum thunb leave extract[A];[C];2007
2 PENG Runling~1 XU Chenghai~2 ZHANG Shiwei~2 LI Quanshun~2 Liu Changyong~1(1 College of Mechanical Engineerin,Shenyang Ligong University,Shenyang 110168,Liaoning,China;2 School of Mechanical Engineering & Automatization,Northeastern University,Shenyang110004,Liaoning,China );Experimental study on freeze-drying of Spirulina platensis[A];Chinese association of Refrigeration[C];2008
Chinese Journal Full-text Database 10 Hits
1 ZHANG Chun_ling, HU Jun_feng, QU Jiang_bin, et al.(School of Public Health, Shandong University, Jinan 250012 , Ch ina);STUDY ON ANTIMUTAGENICITY OF CHINESE HERBAL MEDICINE AND GREEN TEA TO BENZOAPYRENE AND NNK[J];Carcinogenesis,Teratogenesis and Mutagenesis;2003-02
2 Li Fuxiang(Qinghai Normal university biotechnology department 810008);Current Status and Progress of Pharmacological Research in Cistanche[J];Anhui Agricultural Science Bulletin;2006-13
3 MA Shu-feng et al (College of Food Science,Shenyang Agricultural University,Shenyang,Liaoning 110161);Research Advances in Polysaccharides from Pleurotus nebrodensis[J];Journal of Anhui Agricultural Sciences;2005-07
4 LI Feng et al(Biotechnology and Science College,Shenyang Agricultural University,Shenyang,Liaoning 110161);Study on Elimination Protein from the Roofs of Aralia continentalis Kitag Polysaccharide[J];Journal of Anhui Agricultural Sciences;2006-01
5 HE Ling-ling et al (School of Application Chemical,Shenyang Institute of Chemical Technology,Shenyang,Liaoning 110142);Effect of Deprotein Technique on the Extraction of Polysaccharide from the Leaf of Ilex kudincha C.J.Tseng[J];Journal of Anhui Agricultural Sciences;2006-23
6 XIE Dong hua*, TANG Xiao da, XIA Shu jie, TAN Jian ming,WANG Xiang hui, CAI YongDepartment of Urology,The First Peoples Hospital of Shanghai,Shanghai 200080,P.R.China;Expressions of NF-κB in Human Bladder Cancer and Its Clinical Significance[J];Chinese Journal of Cancer;2002-06
7 WANG Zheng-gang (Jiangsu Institute of Microbiology,Wuxi 214063);Research Progress in the Derivatives of the Anino Acids Containing Sulphur[J];Amino Acids & Biotic Resources;2001-01
8 ZHANG Xin Sheng\ LI Jin Ting\ XU Cun Shuan (Department of Biology,Henan Normal University,Xinxiang 453002,China);THE CHANGES OF THE PROTEINASE ACTIVITY IN MOUSE SMALL INTESTINE DURING DEVELOPMENT[J];ACTA ZOOLOGICA SINICA;1997-S1
9 LI Ping WANG Yan-hui MA Run-yu (The Key Laboratory of Science and Technology of Controllable Chemical Reactions, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, China);Water extraction conditions of polysaccharide from Fructus Corni[J];Journal of Beijing University of Chemical Technology;2003-01
10 YANG Jian WANG Yan-hui MA Run-yu (The Key Laboratory of Science and Technology of Controllable Chemical Reactions, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, China);Extraction of corn cob xylan by ultrasonic wave[J];Journal of Beijing University of Chemical Technology;2005-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Zhong,YANG Li(Anhui Science and Technology University,Fengyang 233100,China);Extraction of Polysaccharide in Black Glutinous Corn and Comparison of Removing Protein Procedure[J];Journal of Anhui Science and Technology University;2007-06
2 CAO Cheng-yi1,WANG Kai-yu2,WANG Ling2,HUANG Xiao-li2(1.The Fishery Research Institute of Chengdu Academy of Agriculture and Forestry Sciences,Chengdu 610072;2.Fish Disease research center of Sichuan Agriculture University,Yaan,Sichuan 625014);Isolation and Identification of Pathogenic Bacteria Causing Ulcer Disease of Atlantic salmon[J];Freshwater Fisheries;2009-01
3 LIU Chao-liang,SUN Hui,YIN Zhi-liang,LI Feng,ZHANG Jun,WEI Guo-qing,SHAO Yin-gang,ZHOU Yan(College of Life Science,Anhui Agriculture University,Hefei 230036,Anhui,China);Analysis of Available Active Substance in Crude Ethanol Precipitation of Mulberry Syncarp[J];Acta Laser Biology Sinica;2007-05
4 LI Zhi-min,QIU Fang-ping,ZHAO Li-dong,XIE Song-lin(School of Biological Engineering,Changchun University of Technology,Changchun 130012,China);Extraction and purification optimal conditions for lentinan[J];Journal of Changchun University of Technology(Natural Science Edition);2007-04
5 FAN Hai-yan1,2,SUN Dao-hua1,HE Ning1,YANG Kun1,WANG Yuan-peng1,LI Qing-biao1,2*(1.Department of Chemical and Biochemical Engineering,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen,Fujian,China;2.Key Lab for Chemical Biology of Fujian Province,Xiamen University,Xiamen,Fujian,China);Separation and Decoloration of Stachyose from Radix Rehmanniae Libosch[J];Fine Chemicals;2008-11
6 WU Jian-chun,LI Yan(Oncology Department of Shanghai City's Hospital of TCM attached to Shanghai University of TCM,Shanghai 200071,China);Experimental Study Progress on the Mechanism of TCM Anti-lung Cancer Metastasis[J];Journal of Liaoning University of Traditional Chinese Medicine;2009-04
7 ZHOU Jing (College of Life Science, Qufu Normal University, Qufu 273165,China);Research Progress of Pathogenic Extracellular Products[J];Animal Husbandry and Feed Science;2009-01
8 ZHANG Ke-jun1,ZHANG Xiao2(1.Department of General Surgery,Affiliated Hospital of Hainan Medical College Haikou 570102;2.Department of Biochemistry,Guangdong Medical College Zhanjiang 524000,China);Effect and mechanism of 5F on the cell growth of pancreatic carcinoma[J];Journal of Hainan Medical College;2009-09
9 FENG Qi-gin,HU Ai-jun,HU Xiao-hua (College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China);Application of ultrasonic techniques in health oil extraction[J];Cereals & Oils;2009-08
10 YU TING, LIU Dong-wu,,CHEN Zhi-wei;Clinical application and mechanism of Cantharidin and it's Ramification[J];Life Science Instruments;2009-02
China Proceedings of conference Full-text Database 4 Hits
1 Ji Chenfeng1* Chen Xuejun2 Cheng Bingchen1 Li Mingze2 Ji Yubin1 (1.Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin, 150076; 2.Postdoctal Researh of the Institute of Materia Medica, Harbin University of Commerce, Harbin, 150076);Study on the monosaccharide composition analysis of polysaccharides from Asparagus officinalis by HPCE[A];[C];2006
2 ;芦笋多糖提取、单糖组分分析及含量测定[A];[C];2008
3 ZHOUYong,LI Xue,CHEN Li,WANG Qi (College of Traditional Chinese Medicine,Jinlin Agriculture University,Changchun 130118,China);Studied on the Method of Phellinus igniarius' Hyphostroma Polysaccharides Protein-removed[A];[C];2007
4 yanfang (food science and technology of Nanchang university Nanchang 330047);The Isolation and Purification of the Polysaccharides from Lotus Leaf[A];[C];2005
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved