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《Journal of The Fourth Military Medical University》 2001-06
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Protein quantification with coomassie bulliant blue microplate-colormetric

WANG Duo Ning 1, ZHAO Yan Wu2, TIAN FuRong3 1Department of Basic Medicine, Shaanxi Medical College, Xi'an 710068, China, 2Department of Chemistry, Faculty of Preclinical Medicine, 3Department of Radiomedicine, Faculty of Preventive Medicine,  
AIM To develop a rapid and sensitive method of protein quantification assay. METHODS Protein content of bovine serum albumin (BSA) and mice liver homogenate was determinated by microplate colormetric (MPC) in which coomassie brilliant blue (CBB) is used as color reagent. RESULTS Detecting limit of MPC was lower (0.63 μg) than that of macro colormetric (MC). MPC had advantage of rapidity and lesser volume of sample (a few microliter). CONCLUSION MPC is a method of simplicity, rapidity and sensitivity for protein quantification assay especially suitable for the determination of large number of microliter samples.
【CateGory Index】: Q503
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