Therapeutic effect of anisodamine, ANP and CGRP on oleic acid induced acute lung injury in rabbits
JIAN Wen 1, QI Hao Wen 1, LI Zhi Chao 2 1Department of Respiratory Medicine, Xijing Hospital, 2Department of Pathophysiology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710033, China
AIM To investigate the effect and preliminary mechanism of anisodamine (654 2), atrial natriuretic peptide (ANP) and calcitonin gene related peptide (CGRP) on acute lung injury (ALI) rabbits induced by oleic acid. METHODS The rabbits were randomly divided into 6 groups: oleic acid group (OA group), treatment groups and control group. The mean pulmonary arterial pressures (mPAP) at different time points were detected by using right ventricular vessel technique. The concentrations of endothelin 1 (ET 1) in plasma and bronchio alveolar lavage fluid (BALF) at different time points were continuously analysed by radioimmunoassay. In the meantime, PaO 2 and content of lung water were also observed. RESULTS Compared with that in the control group, mPAP, concentrations of ET 1 in plasma and BALF and content of lung water of the oleic acid group markedly increased ( P 0.05, P 0.01), whereas their PaO 2 decreased ( P 0.01). After treatment, mPAP (except 654 2 group), levels of ET 1 and content of lung water decreased ( P 0.05, P 0.01), though varied from group to group, on the other hand, PaO 2 increased ( P 0.01). CONCLUSION Intravenous infusion of 654 2, ANP, CGRP may have beneficial effects on ALI induced by oleic acid.