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《Journal of The Fourth Military Medical University》 2002-13
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Cloning and expression of gene of house dust mite antigen Der p2

SHI Jie Ran 1, LI Yuan 2, QI Hao Wen 1, LI Bie Hu 2, BAI Yin Lan 2, FAN Xiong Lin 2, XUE Ying 2 1Department of Respiratory Disease, Xijing Hospital, 2Department of Microbiology, Faculty of Preclinical Medicine, Fourth Military Med  
AIM To construct and clone the gene of the house dust mite antigen Der p2, which is expressed in E. coli . METHODS The gene fragments were designed and synthesized by the published house dust mite antigen Der p2. Using single chain amplification by PCR and T4 ligase, we prepared the whole Der p2 gene, which was cloned to express vector pProEX HTb for sequence analysis and expression. RESULTS The whole Der p2 gene was constructed withdesigned structure and expressed by IPTG inducement in E.coli . The Der p2 protein was detected to be about M r 17×10 3 by SDS PAGE. The yield account for up to 20% of the total amount of protein. CONCLUSION The constructed Der p2 gene can be expressed in E.coli .
【Fund】: 第四军医大学博士学位论文课题资助项目 (2 0 0 10 0 7)
【CateGory Index】: R346
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