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《Journal of The Fourth Military Medical University》 2003-21
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Pro-apoptotic effect of a truncated human Bid fusion protein on HeLa cells

QIU Xiu Chun 1,3 , YU Cui Juan 1, XU Yan Ming 1, JIA Lin Tao 1, CAO Yun Xin 2, WANG Zhi 1, WANG Cheng Ji 1, FAN Qing Yu 3, YANG An Gang 1 1Department of Biochemistry and Molecular Biology, 2Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi’an 710033, China, 3Orthopaedic Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China  
AIM: To investigate the pro apoptotic effect of a truncated human Bid fusion protein on HeLa cells. METHODS: A full length bid gene was obtained by RT PCR. Then the full length bid gene was truncated by deleting its N terminal fragment to get a truncated bid (t bid ) gene, which was then fused with partial translocation domain sequence of pseudomonas exotoxin A (PE) to get a fusion protein gene. Both t bid gene and its fusion protein gene were respectively inserted into the pcDNA3 eukaryotic expression vectors , and transfected into HeLa cells. The apoptotic changes in the transfected cells were detected by indirect immunofluorescence technique, electron microscopy and flow cytometry analyses. RESULTS: The products, including full length bid gene, the genes and expression vectors of t bid and its fusion protein with part of the translocation domain of PE were successfully obtained. Compared with the control group, the expression of both genes displayed typical characteristics of apoptosis and had similar effects on HeLa cells. CONCLUSION: Both tBid and its fusion protein can effectively induce apoptosis of HeLa cells.
【Fund】: 国家杰出青年科学基金 (399Z50 36) ;; 国家高技术“863”计划(2 0 0 1AA2 1 71 0 1 )
【CateGory Index】: R346
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【References】
Chinese Journal Full-text Database 8 Hits
1 ZHOU Fu Ling 1, HUANG Chen 2, SONG Tu Sheng 2, NI Lei 2, WANG Ai Ying 2, NIU Yue Ying 2, LI Ming Zhong 1 1Department of Oncologic Radiotherapy, First Hospital, 2Department of Biology, Xi’an Jiaotong University, Xi’an 710061, China;Effects of indole-3-acetic acid oxidated by horseradish peroxidase on proliferation and MDA level of HeLa cells[J];Journal of The Fourth Military Medical University;2004-17
2 WANG Fang~1, QIU Xiu-Chun~3, WANG Li-Feng~1, XU Yan-Ming~1, ZHAO Jing~1, MENG Yan-Ling~2, JIA Lin-Tao~1, WANG Cheng-Ji~1, FAN Qing-Yu~3, YANG An-Gang~2 ~1Department of Biochemistry and Molecular Biology, ~2Department of Immunology, School of Basic Medicine, Xi'an 710033, China, ~3Department of Orthopaedics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China;Expression and proapoptotic activity of recombinant anti-HER2 ScFv/tbid gene in SKBr-3 cells[J];Journal of the Fourth Military Medical University;2006-12
3 JI Zhen-gang1,QIU Xiu-chun1,YANG Tong-tao1,LONG Hua1,MA Bao-an1,ZHOU Yong1,ZHANG Ming-hua1,XU Yan-ming2,YANG An-gang3,FAN Qing-yu1(1.Center of Orthopaedis Surgery,Tangdu Hospital,the Fourth Military Medical University,Xi'an 710038,Shaanxi,China;2.Department of Biochemistry and Molecular Biology,the Fourth Military Medical University,Xi'an 710032,Shaanxi,China;3.Department of Immunology,the Fourth Military Medical University,Xi'an 710032,Shaanxi,China);The pro-apoptotic effect of HER2 targeted recombinant truncated Bid fusion protein on osteosarcoma cells[J];Journal of Medical Postgraduates;2007-02
4 WANG Fang WANG Li-feng QIU Xiu-chun XU Yan-ming BAO Wei MENG Yan-ling WANG Cheng-ji FAN Qing-yu YANG An-gang (Department of Biochemistry and Molecular Biology Xi'an 710032 ,China) (Department of Immunology, the Fourth Military Medical University Xi'an 710032, China) (Orthopaedic Oncology Institute of Chinese PLA, Tangdu Hospital, Fouth Military Medical University Xi'an 710038, China );Investigation of Apoptosis of the SGC7901 Cells Induced by the Expression of the Recombinant Gene of anti-HER2 ScFv/tBid[J];China Biotechnology;2006-04
5 QIU Xiu-chun~1, XU Yan-ming~2,YU Cui-juan~3,ZHAO Jing~2,MENG Yan-ling~3,YANG Tong-tao~1, LONG-Hua~1,MA Bao-an~1, ZHOU-Yong~1,WANG Cheng-ji~2, YANG An-gang~3, FAN Qing-yu~1 ~1Orthopaedic Oncology Institute, Tangdu Hospital, ~2Department of Biochemistry and Molecular Biology, ~3Department of Immunology,Faculty of Preclinical Medicine,Xi’an 710032,China; Fourth Military Medical University, Xi'an 710038, China;Construction of recombinant immuno-tBid gene and expression of a secreted fusion protein in HeLa cells[J];Journal of Modern Oncology;2006-11
6 JI Zhen-gang1,QIU Xiu-chun1,YANG Tong-tao1,LONG Hua1,Zhou Bengen1,Wen Yan-hua1,XU Yan-ming2,YANG An-gang3,FAN Qing-yu11Orthopaedic Oncology Institute,Tangdu Hospital,Xi'an 710038,China;2Department of Biochemistry and Molecular Biology,3Department of Immunology,Faculty of Preclinical Medicine,Fourth Military Medical University,Xi'an 710032,China.;Expression of tBid gene and it' s pro-apoptotic effect on osteosarcoma SOSP-9607 cells[J];Journal of Modern Oncology;2007-05
7 SHEN Jun,DU Lili,ZHAI Aimin(Qingdao Commercial Staff-worker Hospital,Qingdao 266011,China) WANG Zhaoyan(Qingdao Municipal Hospital,Qingdao 266011,China) ZHANG Xiao,GE Yinlin(Medical College of Qingdao University,Qingdao 266021,China) QIU Wensheng(The Affiliated Hospital of Medical College of Qingdao University,Qingdao 266003,China);Induction of Apoptosis by Tanshinone Ⅱ_A in HeLa Cells[J];China Pharmacy;2007-27
8 QIU Xiu-chun1, SHAN Le-qun1, JI Zhen-gang1, YANG Tong-tao1, LONG Hua1, XU Yan-ming2, ZHOU Yong1, MA Bao-an1, YANG An-gang3, FAN Qing-yu1 (1. Department of Orthopedics, Tangdu Hospital; 2. Department of Biochemistry and Molecular Biology, Faculty of Preclinical Medicine; 3. Department of Immunology, Faculty of Preclinical Medicine, the Fourth Military Medical University, Xi′an 710038, China);Pro-apoptotic effect of recombinant anti-HER2 fusion protein ScFv/tBid gene on osteosarcoma E10 cells[J];Chinese Journal of Cancer Biotherapy;2008-02
【Citations】
Chinese Journal Full-text Database 5 Hits
1 JIA Lin Tao, YU Cui Juan, XU Yan Ming, ZHU Feng, PENG Wei Dan, SU Cheng Zhi, WANG Cheng Ji, YANG An Gang Department of Biochemistry and Molecular Biology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710033, China;Construction and effect of caspase-3 fusion proteins and their apoptosis induction in HeLa cells[J];Journal of The Fourth Military Medical University;2001-12
2 ZHAO Jing 1, XU Yan Ming 1, JIA Lin Tao 1, YU Cui Juan 1, CHEN Lei 2, HUANG Wei Quan 2, WANG Cheng Ji 1, YANG An Gang 1 1Department of Biochemistry and Molecular Biology, 2Department of Histology and Embryology, Faculty of Precl;Expression of human granzyme B gene in HeLa cells[J];Journal of The Fourth Military Medical University;2002-05
3 ZHANG Geng 1, WANG He 1, ZHANG Bo 1, YU Cui Juan 2, WU Guo Jun 1, QIN Wei Jun 1, MENG Yan Ling 2, WANG Cheng Ji 2, YANG An Gang 2 1Department of Urology Surgery, Xijing Hospital, 2Department of Biochemistry & Molecular Biology,Faculty of Preclinical Medicine, Fourth Military Medical University, Xian 710033, China;Overexpression of prostate-specific antigen promoter-driven Pro-caspase-7 for the induction of therapeutic apoptosis in prostate cancer[J];Journal of The Fourth Military Medical University;2003-08
4 YU Cui Juan, MENG Yan Ling,GUI Jun Hao,ZHAO Jing, JIN Ming,WANG Zhi,WANG Cheng Ji,YANG An Gang ** (Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xian 710032, China);Gene Construction,Expression and The Apoptosis Inducing Effects on HeLa Cells of The Recombinant Apoptosis Inducing Factor[J];Progress In Biochemistry and Biophysics;2002-06
5 ZHAO Jing1,JIA Lin-tao1, XU Yan-ming1, JIN Ming1, ZHANG Miao-li2, LI Ji-shuo2, WANG Cheng-ji1, YANG An-gang1 1Department of Biochemistry and Molecular Biology, 2Department of Anatomy, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, Ch;The effect of human granzyme B -PE domain Ⅱfusion protein on transfected HeLa cells[J];Journal of Cellular and Molecular Immunology;2002-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 GUI Jun-Hao1, XU Yan-Ming2, YU Cui-Juan2, ZHAO Jing2, JIA Lin-Tao2,WANG Cheng-Ji2, YANG An-Gang2, QIU Dong-Hui1, YU Wu-Zhong1, HE Jiang11. Clinical Medical Research Institute, Urumqi General Hospital, Lanzhou Command, Urumqi, Xinjiang, 830000, P. R. China2. Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi'an, Shaanxi, 710033, P. R. China;Pro-apoptotic Efficiencies of Three Reconstructed Human caspase-8 on Cervical Cancer Cell Line HeLa[J];Chinese Journal of Cancer;2005-02
2 YNAG Xiao-huai1,LI Qing-wen1,ZHANG Jia-jun1,CHENG Ze-nong2(1.Department of Urology,Affiliated Hospital of Medical College,Bengbu 233004;2.Department of Pathology,Bengbu Medical College,Bengbu 233003,China);Expression of Caspase-3 in bladder transitional carcinoma and its significance[J];Journal of Bengbu Medical College;2005-04
3 YU Bing, FAN Qing-yu, YAN Lu,et al. Orthopedics Oncology Institute of PLA, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China;The Apoptosis Induction Effect of Recombinant Caspase-3 on the Human Osteosarcoma Cell Line SOSP-9901[J];Journal of Chinese Physician;2004-12
4 ZHANG Yan Ling 1, CHEN Kang Ning 1, SHAO Shu Qin 1, WANG Ying 2 1Department of Neurology, Southwest Hospital, 2Department of Pathogenic Biology, College of Medicine, Third Military Medcial University, Chongqing 400038, China;Relationship between apoptosis and expression of Caspase-3 after cerebral hemorrhage[J];Journal of The Fourth Military Medical University;2004-01
5 WANG Li-Feng 1, XU Yan-Ming 1, JIA Lin-Tao 1, ZHAO Jin 1, WEN Wei-Hong 2, JIN Ming 1, WANG Cheng-Ji 1, YANG An-Gang 1,2 1Department of Biochemistry and Molecular Biology, 2Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi’an 710033, China;Expression of human perforin in HepG-2 cells[J];Journal of The Fourth Military Medical University;2004-23
6 LIU Xiang-Li 1,YU Cui-Juan 2,XU Yan-Ming 1,ZHAO Jing 1,WANG Cheng-Ji 1,YANG An-Gang 1,2 1Department of Biochemistry & Molecular Biology, 2Department of Immunology,School of Basic Medicine,Fourth Military Medical University ,Xi'an 710033,China;Effect of truncated human apoptosis-inducing factor expression on apoptosis of HeLa cells[J];Journal of The Fourth Military Medical University;2005-06
7 YU Xiao-Lin, LIU Hong, CHEN Wan-Ping, CHENG Peng-YuanOutpatient Department, Fourth Military Medical University, Xi'an 710033, China;Effects of recombinant Caspase-3 on human laryngocarcinoma cell line Hep-2 through coexpression with GFP[J];Journal of The Fourth Military Medical University;2005-21
8 ZHANG Wei, ZHANG Yong, MENG Yan-Ling, WEN Wei-Hong, XUE Qian, REN Jun-Lin, YANG An-GangDepartment of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710033, China;Pro-apoptotic activity of truncated human apoptosis-inducing factor on HeLa cells[J];Journal of the Fourth Military Medical University;2007-07
9 ZHAO Jun-Xia, ZHENG Li-Fen, ZHAO Juan, SHEN Yuan, WANG Yan-Ling, YAN Yun-Li Department of Cell Biology, Basic Medical College, Hebei Medical University, Shijiazhuang, 050017, China;Effects of pholiota nameko polysaccharides on K562 leukemia cell proliferation and Caspase-3 expression in vitro[J];Journal of the Fourth Military Medical University;2007-15
10 WANG xiao-xia1,2,YAO xiao-bao1, JI xian-sheng2, ZHU hong-liang1 1Department of Otolaryngology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China;2Department of Otolaryngology, 451 Hospital of PLA, Xi'an 710054, China;Effect of recombinant caspase-3 gene transfection on CNE2 cell apoptosis[J];Journal of Southern Medical University;2009-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 DENG You ping, LIN Chen, ZHANG Xue yan, et al.National Laboratory of Molecular Oncology,Cancer Institute,Chinese Academy of Medical Sciences,Peking Union Medical College, Beijing 100021, P.R. China;Studies on arsenic trioxide induced human pulmonary adenocarcinoma GLC-82 cell apoptosis and its molecular mechanisms[J];CHINESE JOURNAL OF CANCER;1999-05
2 Xu Yang1, He Bin2, Wang Ya- Jie1, Fu Qiang1 1. Department of Oncology, Changhai Hospital,The second Military Medical University, Shanghai,200433, P.R.China 2. Department of Cardiothoracic Surgery,Changhai Hospital,The Second Military Medical University, Shanghai,200433, P.R.China;Effect of Herceptin Combined with Doxorubicin on Rat Cardiotoxicity[J];Chinese Journal of Cancer;2004-04
3 SONG Jin-Lian, YUE Wang, HOU Lin, GE Yin-LinDepartment of Biochemistry and Molecular Biology, Medical College, Qingdao University, Qingdao, Shandong, 266021, P. R. China;Effects of Isatin on Cell Cycle Arrest and Apoptosis of Neuroblastoma Cell Line SH-SY5Y[J];Chinese Journal of Cancer;2008-03
4 ZHU ZHAO-YI,HOU LIN,JU CHUAN-XIA, et al (Department of Biochemistry,Qingdao University Medical College,Qingdao 266021,China);STUDY OF THE ANTI-PROLIFERATION EFFECTS OF 2,3-DIOXOINDOLINE ON THE SH-SY5Y CELL LINES[J];Acta Academiae Medicinae Qingdao Universitatis;2007-02
5 YU Shao-juan,ZHAO Xue-zhong,ZHANG Guo-cheng,JI Li~(Department of Cadre Ward,First Hospital,Jilin University,Changchun 130021,China);Apoptosis of cardiocytes of neonatal rats with simulated ischemia/reperfusion injury mediated by pro-apoptosis by protein Bid[J];Journal of Jilin University(Medicine Edition);2006-02
6 HAO Xin-Bao, FAN Qing-Yu, ZHANG Dian-Zhong, QIU Xiu-Chun, YIN Jian-Ning, ZHANGHui-Zhong, WANG Xiao-Ning(Orthopedics Oncology Institute of Chinese PLA,Tangdu Hospital, Fourth Military Medical Univer-sity, Xi'an 71OO38, China);Immunotherapy of rat osteosarcoma using tumor vaccine from fusion of macrophages with osteosarcoma cells[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-12
7 YANG Xing Long 1, ZHU Ming 2, MIAO Lu 3 1Medical& Educational Administration,Xijing Hospital,Xi’an 710033,China, 2Department of Otorhinolaryngology,Zhujiang Hospital,First Military Medical University, 3Research Administration,Four;Quantitative changes of microvasculature during multistage morphogenesis of nasopharyngeal carcinoma[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-02
8 LI Yang 1, XIONG Li Ze 1, QIN Xu Jun 2, CHEN Shao Yang 1, WANG Qing De 1 1Department of Anesthesiology, Xijing Hospital, 2Department of Toxicology, School of Preventive Medicine, Fourth Military Medical University, Xian 710033, China;Effect of Shenfu injection on the superoxide dismutase activities and malondialdehyde levels in sera of supratentorial cerebral tumor patients during operation[J];Journal of The Fourth Military Medical University;2003-16
9 WANG Hong-mei, ZHANG Gui-yingDepartment of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410078, China;Experimental study of the inhibitory effect of indomethacin on the growth and angiogenesis of human colon cancer xenografts[J];Journal of First Military Medical University;2004-02
10 Dai Zhu,Hu Jianzhong,Lü Hongbin,et al.Department of Orthopedics,the 1st Affiliated Hospital of Nanhua University, Hengyang,421001;Expression of Bid Protein in Osteoarthrosis Articular Chondrocytes and Its Possible Significance[J];Chinese Journal of Bone and Joint Injury;2006-10
【Secondary References】
Chinese Journal Full-text Database 9 Hits
1 LIU Li-Ying 1,2, SONG Tu-Sheng1, HUANG Chen 1,2, YANG Ling 1,2, LUO Yu2, NI Lei1, SI Lü-Sheng3 1Department of Biology & Genetics, 2Key Laboratory of Environment & Disease Correlation Gene, 3Institute of Immunopathology, College of Life Science and Technology, Xi'an Jiaotong University, Xi' an 710061, China;Effects of indole-3-acetic and horseradish on apoptosis of BXPC-3 cells[J];Journal of The Fourth Military Medical University;2005-15
2 YUAN Chun-yi,XIE Qiong,Director: ZENG Bai-rong,HE Xin The Master of Hunan University of Traditional Chinese Medicine,Changsha,China,410007;Experimental Study Progress of TanshinoneⅡA in Tumor Bound[J];Guiding Journal of Traditional Chinese Medicine and Pharmacy;2009-09
3 SHEN Ping1,ZHANG Fang 1,WU Xue-ling2,SHI Yi1,QIAN Gui-sheng2,LUO Xiang-dong3,HU Bo1,SONG Yong1(1.Department of Respiration Diseases,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 210002,Jiangsu,China; 2.Insititute of Respiration Diseases,Xinqiao Hospital,Chongqing 400037,China; 3.Burn Institute,Xinan Hospital,Third Military Medical University,Chongqing 400037,China);Inhibition of A549 cell proliferation by RNA interfered gene her2/neu[J];Journal of Medical Postgraduates;2009-03
4 YE Yin-tao,XU Wen-qing,SHEN Xiu,et al. Institute of Radiation Medicine,Chinese Academy of Medical Sciences,Tianjin 300192 China.;The Radiosensitizing Effects of Dihydrotanshinone Ⅰ on H22 Hepatoma-bearing Mices[J];Chinese Journal of Radiological Health;2009-03
5 CHU Yan-qi,ZHANG Lan,WANG Wen,LI Wei,ZHANG Li,LI Lin(Dept.of Pharmacology,Xuanwu Hospital of Capital Medical University;Key Laboratory for Neurodegenerative Diseases of Ministry of Ed-ucation,Beijing 100053,China);Anti-apoptotic Effects of Cornus Officinals Iridoid Glycoside on Cellular Model of Alzheimer Disease Induced by Protein Phosphatase Inhibitor Okadaic Acid[J];China Pharmacy;2009-18
6 LIU Chuan-yu,TANG Jian-hong,SU Han,ZHANG Jian-yi(The Affiliated Hospital of Guilin Medical College,Guilin 541001,China);Effects of Yixinshu Capsule on Cardiac Function and Plasma Levels of Ang Ⅱ and TNF-α in Patients with Chronic Cardiac Failure[J];China Pharmacy;2009-30
7 YE Yintao,XU Wenqing,ZHONG Wei(Institute of Radiation Medicine,Chinese Academy of Medical Sciences,TianjinKey Laboratory of Molecular Nuclear Medicine,Tianjin 300192,China);Effects of cryptotanshinone on proliferation and apoptosis of Hela cell line of cervical cancer[J];China Journal of Chinese Materia Medica;2010-01
8 ZHU Min1,2,CHEN Ping1,CHEN Dan1,2,et al 1Renmin Hospital of Yunyang Medical College(Shiyan 442000) 2Hubei Traditional Chinese Medicine College(Wuhan 430000);The Effects of Inhibition and Inducing Apoptosis of Tanshinone Ⅱ A on Human Ovarian Cancer A2780 Cell Lines[J];Journal of Emergency in Traditional Chinese Medicine;2009-04
9 GAO Guo-hui1,WANG Jin-dan1,HUANG Qi-di2,YANG Ji-feng1,YANG Shui-bing1,BAO Bing-bing3,ZHANG Yu3,HU Xiao-qu2(1School of Life Sciences,2Department of Surgical Oncology,The First Affiliated Hospital,3Renji School,Wenzhou Medical College,Wenzhou 325000,China);Expression of fusion protein anti-HER2-ScFv-GFP in insect cells and binding to surface of breast cancer cells[J];Chinese Journal of Pathophysiology;2011-05
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