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Effects of basic fibroblast growth factor on proliferation and differentiation of human periodontal ligament cells in vitro

WANG Yu 1, QIAN Feng Lan 2, WANG Ping 1, YANG Ya Ning 1, HAO Jian Jun 1 1Department of Conservative Densitry, School of Stomatology, Fourth Military Medical University, Xi'an 710033, China, 2Department of Clinical Laboratory, Chengdu Political Fire Brigade Hospital, Chengdu 610042, China  
AIM: To evaluate the effects of human basic fibroblast growth factor (hbFGF, 50 μg·L -1 ) on the proliferation and differentiation of human periodontal ligament cells (PDLC) in vitro . METHODS: The number of cells was counted at different time (3, 9, 15 and 21 d) after adding hbFGF into the culture medium and HE and immunohistochemistry staining were conducted. RESULTS: hbFGF (50 μg·L -1 ) stimulated the cells proliferation and didn't change the morphology and growth mode of the cells. There was no significant difference in expression of ConA and WGA receptors between the two groups and no mineralized extracellar matrix of cells was observed. CONCLUSION: hbFGF may stimulate the proliferation of human periodontal ligament cells in vitro, but no effects on the phenotype of cells have been obseved in this experiment.
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