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Determination of Fluconazol Eye Drops by fluorospectrophotometry

BAI Jian Wei 1, JIN Yuan Chao 2, BAI Lin 3, JIN Wei 4 1Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi’an 710033, China, 2Department of General Surgery, 3Department of Pharmacy, General Hospital of Chinese PLA, Beijing 100853, China, 4The First Cadet Battalion, Brigade of Cadets, First Military Medical University, Guangzhou 510515, China  
AIM: To determine Fluconazol Eye Drops by fluorospectrophotometry. METHODS: The excitation wavelength was 261 nm, emission wavelength 284 nm and bandpass 5 nm. The PM gain was normal and time scanning speed was 10 nm/min. RESULTS: The Fluconazol was linear in the range of 0.002-0.026 g/L for Fluconazol ( r = 0.9998). The recovery rate for the three concentration were 99.4%, 100.6% and 100.8% respectively, and the relative standard deviations (RSD) of the method were 0.38%, 0.63% and 0.56% , respectively. CONCLUSION: This method is easy, accurate and reproducible and can be used for the quality control of Fluconazol Eye Drops.
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