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《Journal of The Fourth Military Medical University》 2005-04
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Therapeutic effect of ANP on pulmonary microvascular endothelial cells injury induced by LPS

LI Zhi-Bin,LI Zhi-Chao,YAN Zhi-Qiang,SONG Jing-Chun,ZHAO Peng-Tao Department of Pathophysiology,School of Basic Medicine,Fourth Military Medical University,Xi'an 710033,China  
AIM: To investigate the therapeutic effect of atrial natriuretic peptide (ANP) on pulmonary microvascular endothelial cells (PMVECs) injury induced by lipopolysaccharide (LPS). METHODS: Cultured PMVECs were divided into control group,LPS group and ANP treatment groups (LPS+ANP).The ANP treatment group was subdivided respectively into 3 groups treated with different concentrations of ANP (0.01,0.1 and 1 μmol/L) and 4 groups treated for different time periods before adding ANP (0,6,12 and 24 h after LPS stimulation).The viability of PMVECs was examined by MTT assay and the cell cycle was analyzed by flow cytometry.The LDH activity and MDA production were measured from supernatants.RESULTS: ANP inhibited the decrease of the proportion of cells in S phase and G2-M phase induced by LPS.The A 490 nm value declined and the LDH activity and MDA production increased in LPS groups compared with those in control groups (P0.05). But the A 490 nm value increased and the LDH activity and MDA production reduced in ANP treatment groups compared with those in LPS groups (P0.05) in a dose-and-time dependent manner.CONCLUSION: ANP has some beneficial effects on LPS-inuced PMVECs injury.
【CateGory Index】: R563
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